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Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice.
Shimatani, Zenpei; Fujikura, Ushio; Ishii, Hisaki; Matsui, Yusuke; Suzuki, Minoru; Ueke, Yuki; Taoka, Ken-Ichiro; Terada, Rie; Nishida, Keiji; Kondo, Akihiko.
Afiliación
  • Shimatani Z; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
  • Fujikura U; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
  • Ishii H; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
  • Matsui Y; Graduate School of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, Aichi, 468-8502, Japan.
  • Suzuki M; Graduate School of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, Aichi, 468-8502, Japan.
  • Ueke Y; Graduate School of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, Aichi, 468-8502, Japan.
  • Taoka KI; Kihara Institute for Biological Research, Yokohama City University, 642-12 Maioka, Totsuka, Yokohama, Kanagawa, 244-0813, Japan.
  • Terada R; Graduate School of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, Aichi, 468-8502, Japan.
  • Nishida K; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan. Electronic address: keiji_nishida@people.kobe-u.ac.jp.
  • Kondo A; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan. Electronic addres
Plant Physiol Biochem ; 131: 78-83, 2018 Oct.
Article en En | MEDLINE | ID: mdl-29778643
The CRISPR/Cas9 system is a revolutionary genome-editing tool for directed gene editing in various organisms. Cas9 variants can be applied as molecular homing devices when combined with various functional effectors such as transcriptional activators or DNA modification enzymes. Target-AID is a synthetic complex of nuclease deficient Cas9 fused to an activation-induced cytidine deaminase (AID) that enables targeted nucleotide substitution (C to T or G to A). We previously demonstrated that the introduction of desired point mutations into target genes by Target-AID confers herbicide tolerance to rice callus. Inheritance of the introduced mutations, as well as the removal of transgenes, are key issues that must be addressed in order to fully develop Target-AID as a plant breeding technique. Here we report the transmission of such mutations from the callus to regenerants and their progenies, leading to a generation of selectable marker-free (SMF) herbicide tolerant rice plants with simultaneous multiplex nucleotide substitutions. These findings demonstrate that Target-AID can be developed into novel plant breeding technology which enables improvement of multiplex traits at one time in combination with sophisticated targeted base editing with the simplicity and versatility of CRISPR/Cas9 system.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Plantas Modificadas Genéticamente / Genes de Plantas / Marcación de Gen / Sistemas CRISPR-Cas / Edición Génica Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Plantas Modificadas Genéticamente / Genes de Plantas / Marcación de Gen / Sistemas CRISPR-Cas / Edición Génica Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Francia