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Characterization of the outer membrane proteome of Francisella noatunensis subsp. orientalis.
Shahin, K; Thompson, K D; Inglis, N F; Mclean, K; Ramirez-Paredes, J G; Monaghan, S J; Hoare, R; Fontaine, M; Metselaar, M; Adams, A.
Afiliación
  • Shahin K; Faculty of Natural Sciences, Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK.
  • Thompson KD; Aquatic Animals Diseases Lab, Aquaculture Division, National Institute of Oceanography and Fisheries, Suez, Egypt.
  • Inglis NF; Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, UK.
  • Mclean K; Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, UK.
  • Ramirez-Paredes JG; Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, UK.
  • Monaghan SJ; Faculty of Natural Sciences, Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK.
  • Hoare R; Faculty of Natural Sciences, Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK.
  • Fontaine M; Faculty of Natural Sciences, Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK.
  • Metselaar M; Benchmark Animal Health, Bush House, Edinburgh Technopole, Edinburgh, Midlothian, UK.
  • Adams A; Benchmark Animal Health, Bush House, Edinburgh Technopole, Edinburgh, Midlothian, UK.
J Appl Microbiol ; 125(3): 686-699, 2018 Sep.
Article en En | MEDLINE | ID: mdl-29777634
AIMS: The aims of the current study were to characterize the outer membrane proteins (OMPs) of Francisella noatunensis subsp. orientalis (Fno) STIR-GUS-F2f7, and identify proteins recognized by sera from tilapia, Oreochromis niloticus, (L) that survived experimental challenge with Fno. METHODS AND RESULTS: The composition of the OMPs of a virulent strain of Fno (STIR-GUS-F2f7), isolated from diseased red Nile tilapia in the United Kingdom, was examined. The sarcosine-insoluble OMPs fraction was screened with tilapia hyperimmune sera by western blot analysis following separation of the proteins by 1D SDS-PAGE. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was used to identify the various proteins present in the OMP profile. Two hundred and thirty-nine proteins were identified, of which 44 were found in the immunogenic band recognized by the tilapia hyperimmune serum. In silico analysis was performed to predict the function and location of the OMPs identified by MS. CONCLUSIONS: Using a powerful proteomic-based approach in conjugation with western immunoblotting, proteins comprising the outer membrane fraction of Fno STIR-GUS-F2f7 were identified, catalogued and screened for immune recognition by tilapia sera. SIGNIFICANCE AND IMPACT OF THE STUDY: The current study is the first report on the characterization of Fno-OMPs. The findings here provide preliminary data on bacterial surface proteins that exist in direct contact with the host's immune defences during infection and offer an insight into the pathogenesis of Fno.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Proteoma / Francisella Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Proteoma / Francisella Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido