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Immunohistochemical investigation of the cross-reactivity of selected cell markers in formalin-fixed, paraffin-embedded lymphoid tissues of Franciscana (Pontoporia blainvillei).
Díaz-Delgado, J; Ressio, R; Groch, K R; Catão-Dias, J L.
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  • Díaz-Delgado J; Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, São Paulo, Brazil; Instituto Adolfo Lutz (IAL), Centro de Patologia, Av. Dr. Arnaldo, 351-7 Andar, Sala 706, Pacaembu, São Paulo, Brazil. Electronic address: j
  • Ressio R; Instituto Adolfo Lutz (IAL), Centro de Patologia, Av. Dr. Arnaldo, 351-7 Andar, Sala 706, Pacaembu, São Paulo, Brazil.
  • Groch KR; Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, São Paulo, Brazil.
  • Catão-Dias JL; Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, São Paulo, Brazil.
Vet Immunol Immunopathol ; 200: 52-58, 2018 Jun.
Article en En | MEDLINE | ID: mdl-29776612
A considerable amount of knowledge on natural and anthropogenic pathologic conditions affecting different cetacean species has been gained over the last decades. Nonetheless, the immunopathological bases for most of these processes have been poorly documented or remain unknown. Comparative immunopathological investigations in these species are precluded by the limited number of specific antibodies, most of which are not commercially available, and the reduced spectrum of validated and/or cross-reactive ones. To partially fill in this gap of knowledge, a set of commercially available primary antibodies were tested for cross-reactivity against leukocytes and cytokines in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissues (lymph nodes, spleen and thymus) of three bycaught, apparently healthy and fresh Franciscanas (Pontoporia blainvillei) using immunohistochemistry. On the basis of similar region specificity within the lymphoid organs, cellular morphology and staining pattern with human control tissues, 13/19 primary antibodies (caspase 3, CD3, CD57, CD68, FoxP3, HLA-DRα, IFNγ, IgG, IL4, IL10, Lysozyme, TGFß and PAX-5) exhibited satisfactory cross-reactivity. Our results expand the spectrum of suitable cross-reactive primary antibodies in FFPE cetacean tissues. Further comparative immunopathological studies focused on infectious diseases and ecotoxicology may benefit from establishment of baseline expression of immunologically relevant molecules in various cetaceans species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacciones Cruzadas / Delfines / Tejido Linfoide / Anticuerpos Límite: Animals Idioma: En Revista: Vet Immunol Immunopathol Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacciones Cruzadas / Delfines / Tejido Linfoide / Anticuerpos Límite: Animals Idioma: En Revista: Vet Immunol Immunopathol Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos