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Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses.
Braun, Ueli; Janett, Fredi; Züblin, Sandra; von Büren, Michèle; Hilbe, Monika; Zanoni, Reto; Schweizer, Matthias.
Afiliación
  • Braun U; Department of Farm Animals, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland. ubraun@vetclinics.uzh.ch.
  • Janett F; Department of Farm Animals, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
  • Züblin S; Department of Farm Animals, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
  • von Büren M; Department of Farm Animals, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
  • Hilbe M; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
  • Zanoni R; Institute for Virology and Immunology, and Department of Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Schweizer M; Institute for Virology and Immunology, and Department of Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
BMC Vet Res ; 14(1): 159, 2018 May 16.
Article en En | MEDLINE | ID: mdl-29769076
BACKGROUND: This study examined various health variables in cows after artificial insemination with Border disease virus (BDV)-infected semen and the occurrence of persistent infection in ensuing fetuses. Five cows were inseminated (day 0) with BDV-infected semen as well as with semen from a fertile Eringer bull. One cow, inseminated with virus-free semen only, served as a control. Clinical examination, assessment of eating and rumination activities, measurement of intraruminal temperature and leukocyte count were used to monitor the health of the cows. Blood samples were collected at regular intervals for the detection of viral RNA and antibodies against BDV, and the cows were slaughtered on day 56. The uteri, placentae and fetuses were examined macroscopically, histologically, immunohistochemically and by means of molecular methods for the presence of pestiviruses. RESULTS: The demeanour, eating and rumination activities and intraruminal temperature were not affected by insemination with BDV-infected semen, whereas the total leukocyte and lymphocyte counts dropped transiently and were significantly lower on day 6 than on day 0. Seroconversion occurred by day 28 in the five infected cows but not in the control cow. The uteri, placentae and fetuses had no macroscopic or histological lesions, and immunohistochemical examination and RT-PCR were negative for pestiviruses. CONCLUSIONS: The findings showed that cows inseminated with BDV-infected semen seroconverted and fetuses thus produced were not persistently infected. Transmission of BDV to cattle through infected semen, therefore, seems to be of minor importance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Enfermedad de la Frontera / Inseminación Artificial / Enfermedades de los Bovinos / Virus de la Enfermedad de la Frontera / Enfermedades Fetales / Seroconversión Límite: Animals / Pregnancy Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2018 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Enfermedad de la Frontera / Inseminación Artificial / Enfermedades de los Bovinos / Virus de la Enfermedad de la Frontera / Enfermedades Fetales / Seroconversión Límite: Animals / Pregnancy Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2018 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido