2-Aminopurine and 5-bromouracil induce alleviation of type I restriction in Escherichia coli: mismatches function as inducing signals?

Efimova, E P; Delver, E P; Belogurov, A A

Efimova, E P; Delver, E P; Belogurov, A A.

Afiliación

Efimova EP; National Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.

Mol Gen Genet ; 214(2): 317-20, 1988 Oct.

Article en En | MEDLINE | ID: mdl-2976882

RESUMEN

The EcoK restriction of unmodified phage lambda is 1000-fold alleviated in Escherichia coli grown in the presence of base analogs 2-aminopurine (2AP) and 5-bromouracil (5BU). 2AP treatment of bacteria affects specifically the type I restriction systems (EcoA, EcoB, EcoD and EcoK) and does not influence type II (EcoRI) and type III (EcoP1) restriction. 2AP-induced alleviation of restriction occurs in bacteria which are deficient in the SOS response (recA and lexA) and mismatch repair (mutH, mutL and mutS) and can be distinguished from the alleviation of restriction observed in dam- strains. We suggest that mismatches induced by 2AP and 5BU may function as an inducing signal for the alleviation of restriction observed in the presence of base analogs.

Asunto(s)

2-Aminopurina/farmacología; Adenina/análogos & derivados; Bromouracilo/farmacología; Desoxirribonucleasas de Localización Especificada Tipo I/genética; Escherichia coli/genética; Bacteriófago lambda/genética; Desoxirribonucleasas de Localización Especificada Tipo I/metabolismo; Escherichia coli/efectos de los fármacos; Escherichia coli/enzimología; Genotipo; Fenotipo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bromouracilo / Adenina / Desoxirribonucleasas de Localización Especificada Tipo I / Escherichia coli / 2-Aminopurina Idioma: En Revista: Mol Gen Genet Año: 1988 Tipo del documento: Article Pais de publicación: Alemania

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