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Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions.
Bi, Huihui; Shi, Jianxin; Kovalchuk, Nataliya; Luang, Sukanya; Bazanova, Natalia; Chirkova, Larissa; Zhang, Dabing; Shavrukov, Yuri; Stepanenko, Anton; Tricker, Penny; Langridge, Peter; Hrmova, Maria; Lopato, Sergiy; Borisjuk, Nikolai.
Afiliación
  • Bi H; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Shi J; Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 20040, China.
  • Kovalchuk N; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Luang S; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Bazanova N; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Chirkova L; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Zhang D; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Shavrukov Y; Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 20040, China.
  • Stepanenko A; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Tricker P; School of Life Sciences, Huaiyin Normal University, Huaian, 223300, China.
  • Langridge P; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Hrmova M; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Lopato S; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
  • Borisjuk N; School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia, 5064, Australia.
Plant Cell Environ ; 41(11): 2549-2566, 2018 11.
Article en En | MEDLINE | ID: mdl-29761511
Transcription factors regulate multiple networks, mediating the responses of organisms to stresses, including drought. Here, we investigated the role of the wheat transcription factor TaSHN1 in crop growth and drought tolerance. TaSHN1, isolated from bread wheat, was characterized for molecular interactions and functionality. The overexpression of TaSHN1 in wheat was followed by the evaluation of T2 and T3 transgenic lines for drought tolerance, growth, and yield components. Leaf surface changes were analysed by light microscopy, SEM, TEM, and GC-MS/GC-FID. TaSHN1 behaves as a transcriptional activator in a yeast transactivation assay and binds stress-related DNA cis-elements, determinants of which were revealed using 3D molecular modelling. The overexpression of TaSHN1 in transgenic wheat did not result in a yield penalty under the controlled plant growth conditions of a glasshouse. Transgenic lines had significantly lower stomatal density and leaf water loss and exhibited improved recovery after severe drought, compared with control plants. The comparative analysis of cuticular waxes revealed an increased accumulation of alkanes in leaves of transgenic lines. Our data demonstrate that TaSHN1 may operate as a positive modulator of drought stress tolerance. Positive attributes could be mediated through an enhanced accumulation of alkanes and reduced stomatal density.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Triticum / Hojas de la Planta Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Triticum / Hojas de la Planta Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2018 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos