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Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus.
Hamdy, Mervat E; Del Carlo, Michele; Hussein, Hussein A; Salah, Taher A; El-Deeb, Ayman H; Emara, Mohamed M; Pezzoni, Guilia; Compagnone, Dario.
Afiliación
  • Hamdy ME; Department of Biotechnology Animal Health Research Institute, Agriculture Research Centre, Giza, 12618, Egypt.
  • Del Carlo M; Department of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, 64023, Teramo, Italy.
  • Hussein HA; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt. husvirol@cu.edu.eg.
  • Salah TA; Nanotechnology Research Centre, British University, Cairo, Egypt.
  • El-Deeb AH; Nanotechnology and Advanced Materials Central Lab, Agricultural Research Centre, Giza, Egypt.
  • Emara MM; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
  • Pezzoni G; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
  • Compagnone D; Department of Biotechnology, Zooprofilattico Institute of Lombardy and Emilia Romagna (IZSLER), 25124, Brescia, Italy.
J Nanobiotechnology ; 16(1): 48, 2018 May 11.
Article en En | MEDLINE | ID: mdl-29751767
BACKGROUND: Nano-PCR is a recent tool that is used in viral diseases diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the diagnosis of different diseases including viral diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV. RESULTS: The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivity of the AuNPs-FMDV biosensor was 10 copy number RNA standards in RT-PCR and 1 copy number RNA standard in real-time rRT-PCR with a 94.5% efficiency, 0.989 R2, a - 3.544 slope and 100% specificity (no cross-reactivity with SVDV). These findings were confirmed by the specific and sensitive recognition of 31 Egyptian FMDV clinical isolates that represents the three FMDV serotypes (O, A, and SAT2). CONCLUSIONS: The AuNPs-FMDV biosensor presents in this study demonstrates a superior analytical and clinical performance for FMDV diagnosis. In addition, this biosensor has a simple workflow and accelerates epidemiological surveillance, hence, it is qualified as an efficient FMDV diagnosis tool for quarantine stations and farms particularly in FMDV endemic areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanopartículas del Metal / Fiebre Aftosa / Oro Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals Idioma: En Revista: J Nanobiotechnology Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanopartículas del Metal / Fiebre Aftosa / Oro Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals Idioma: En Revista: J Nanobiotechnology Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido