Streptozotocin-induced diabetes alters transcription of multiple genes necessary for extracellular matrix remodeling in rat patellar tendon.

Patel, Shivam H; Sabbaghi, Arman; Carroll, Chad C

Patel, Shivam H; Sabbaghi, Arman; Carroll, Chad C.

Afiliación

Patel SH; a Department of Health and Kinesiology , Purdue University , West Lafayette , IN , USA.
Sabbaghi A; b Department of Statistics , Purdue University , West Lafayette , IN , USA.
Carroll CC; a Department of Health and Kinesiology , Purdue University , West Lafayette , IN , USA.

Connect Tissue Res ; 59(5): 447-457, 2018 09.

Article en En | MEDLINE | ID: mdl-29745261

RESUMEN

OVERVIEW: Tendon collagen fibril degradation is commonly seen in tendons of diabetics, but the mechanisms responsible for these changes remain to be elucidated. We have demonstrated that streptozotocin (STZ)-induced diabetes increases tendon cell proliferation and collagen content. In the present study, we evaluated that impact of STZ-induced diabetes on mRNA transcripts involved with collagen fibril organization, extracellular matrix (ECM) remodeling, apoptosis, and proliferation. MATERIALS AND METHODS: Rats were divided into four groups: nondiabetic (control, n = 9), 1 week (acute, n = 8) or 10 weeks of diabetes (chronic, n = 7), and 10 weeks of diabetes with insulin (insulin, n = 8). RNA was isolated from the patellar tendon for determination of mRNA transcripts using droplet digital PCR (ddPCR). RESULTS: Transcripts for Col1a1, Col3a1, Mmp2, Timp1, Scx, Tnmd, Casp3, Casp8, and Ager were lower in acute relative to control and insulin rats (p ≤ 0.05). With the exception of Scx, transcripts for Col1a1, Col3a1, Mmp2, Timp1, Tnmd, Casp3, Casp8, and Ager were also lower in chronic when compared to control (p < 0.05). Transcripts for Col1a1, Col3a1, Mmp2, Timp1, Tnmd, Casp3, Casp8, and Ager were not different between control and insulin (p > 0.05). Transcripts for Dcn, Mmp1a, Mmp9, Pcna, Tgfbr3, Ptgs2, Ptger2, Ptges, and iNos were not altered by diabetes or insulin (p > 0.05). CONCLUSION: Our findings indicated that STZ-induced diabetes results in rapid and large changes in the expression of several genes that are key to ECM remodeling, maintenance, and maturation.

Asunto(s)

Diabetes Mellitus Experimental/genética; Diabetes Mellitus Experimental/patología; Matriz Extracelular/metabolismo; Ligamento Rotuliano/metabolismo; Ligamento Rotuliano/patología; Transcripción Genética; Animales; Colágeno Tipo I/genética; Colágeno Tipo I/metabolismo; Colágeno Tipo III/genética; Colágeno Tipo III/metabolismo; Regulación de la Expresión Génica; Masculino; Metaloproteinasas de la Matriz/genética; Metaloproteinasas de la Matriz/metabolismo; ARN Mensajero/genética; ARN Mensajero/metabolismo; Ratas Sprague-Dawley

Palabras clave

Diabetes; RNA; extracellular matrix; rat; tendon; transcription

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Ligamento Rotuliano / Diabetes Mellitus Experimental / Matriz Extracelular Límite: Animals Idioma: En Revista: Connect Tissue Res Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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