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Bactericide effect of methylene blue associated with low-level laser therapy in Escherichia coli bacteria isolated from pressure ulcers.
Gomes, Thais Ferreira; Pedrosa, Matheus Masalskiene; de Toledo, Ana Claudia Laforga; Arnoni, Veridiana Wanshi; Dos Santos Monteiro, Mirian; Piai, Davi Cury; Sylvestre, Silvia Helena Zacarias; Ferreira, Bruno.
Afiliación
  • Gomes TF; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil.
  • Pedrosa MM; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil.
  • de Toledo ACL; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil.
  • Arnoni VW; Study Center Collucci, Collucci's Clinical, Ribeirão Preto, São Paulo, Brazil.
  • Dos Santos Monteiro M; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil.
  • Piai DC; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil.
  • Sylvestre SHZ; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil.
  • Ferreira B; Department of Physical therapy, University Center UNIFAFIBE, Bebedouro, São Paulo, Brazil. brunof22@me.com.
Lasers Med Sci ; 33(8): 1723-1731, 2018 Nov.
Article en En | MEDLINE | ID: mdl-29744751
The present study analyzed the bactericidal effect of methylene blue associated with low-level lasers on Escherichia coli isolated from a pressure ulcer. Microbiological material from a pressure ulcer was isolated using an aseptic swab, and antimicrobial activity was verified using the diffusion disc method. Methylene blue was used at concentrations of 0.001 and 0.005%, and low-level lasers of 670, 830, and 904 nm, with the energy densities of 4, 8, 10, and 14 J/cm2, were tested on three plates each and combined with methylene blue of each concentration. In addition, three control plates were used, with each concentration and energy density separated without any interventions. The results were analyzed using the paired sample t test to determine the bactericidal effect of the methylene blue and using the ANOVA test to compare the effects of the energy densities and wavelengths among the low-level laser treatment protocols. The results showed bacterial reduction at wavelengths of 830 and 904 nm and more proliferation in wavelengths of 670 nm. In wavelength of 830 nm, a bacterial reduction was observed in the conditions with 0.001% methylene blue in all energy density utilized, with 0.005% methylene blue in energy density of 10 J/cm2, and without methylene blue in energy density at 10 J/cm2. And in a wavelength of 904 nm, all condition showed bacterial reduction with or without methylene blue. We concluded that the low-level lasers of 904 and 830 nm have bactericidal effects and at better energy densities (10 and 14 J/cm2).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terapia por Luz de Baja Intensidad / Úlcera por Presión / Escherichia coli / Azul de Metileno / Antibacterianos Tipo de estudio: Guideline / Risk_factors_studies Límite: Humans Idioma: En Revista: Lasers Med Sci Asunto de la revista: BIOTECNOLOGIA / RADIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terapia por Luz de Baja Intensidad / Úlcera por Presión / Escherichia coli / Azul de Metileno / Antibacterianos Tipo de estudio: Guideline / Risk_factors_studies Límite: Humans Idioma: En Revista: Lasers Med Sci Asunto de la revista: BIOTECNOLOGIA / RADIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido