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[Effects of legume-oat intercropping on abundance and community structure of soil N2-fixing bacteria]. / 豆科作物间作燕麦对土壤固氮微生物丰度和群落结构的影响.
Yang, Ya Dong; Feng, Xiao Min; Hu, Yue Gao; Ren, Chang Zhong; Zeng, Zhao Hai.
Afiliación
  • Yang YD; College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
  • Feng XM; College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
  • Hu YG; Institute of Crop Science, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, Beijing 100081, China.
  • Ren CZ; College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
  • Zeng ZH; Baicheng Academy of Agricultural Sciences, Baicheng 137000, China.
Ying Yong Sheng Tai Xue Bao ; 28(3): 957-965, 2017 Mar 18.
Article en Zh | MEDLINE | ID: mdl-29741025
In this study, real-time PCR and high-throughput sequencing approaches were employed to investigate the abundance and community structure of N2-fixing bacteria in a field experiment with three planting patterns (Oat monoculture, O; Soybean-oat intercropping, OSO; Mung bean-oat intercropping, OMO). The results showed that soil chemical properties varied significantly in different soil samples (P＜0.05). The abundance of nifH gene varied from 1.75×1010 to 7.37×1010 copies·g-1 dry soil in all soil samples. The copy numbers of nifH gene in OSO and OMO were 2.18, 2.64, and 1.92, 2.57 times as much as that in O at jointing and mature stages, with a significant decline from jointing to mature stage for all treatments (P＜0.05). Rarefaction curve and cove-rage results proved the nifH gene sequencing results were reliable, and the diversity index showed that the N2-fixing bacteria diversity of OSO was much higher than that of O. Azohydromonas, Azotobacter, Bradyrhizobium, Skermanella and other groups that could not be classified are the dominant genera, with significant differences in proportion of these dominant groups observed among all soil samples (P＜0.05). Venn and PCA analysis indicated that there were greater differences of nifH gene communities between jointing and mature stages; however, the OSO and OMO had similar communities in both stages. All these results confirmed that legume-oat intercropping significantly increased the abundance and changed the community composition of N2-fixing bacteria in oat soils.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Suelo / Fabaceae Idioma: Zh Revista: Ying Yong Sheng Tai Xue Bao Asunto de la revista: SAUDE AMBIENTAL Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Suelo / Fabaceae Idioma: Zh Revista: Ying Yong Sheng Tai Xue Bao Asunto de la revista: SAUDE AMBIENTAL Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: China