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The role of type I interferons (IFNs) in the regulation of chicken macrophage inflammatory response to bacterial challenge.
Garrido, Damien; Alber, Andreas; Kut, Emmanuel; Chanteloup, Nathalie K; Lion, Adrien; Trotereau, Angélina; Dupont, Joëlle; Tedin, Karsten; Kaspers, Bernd; Vervelde, Lonneke; Trapp, Sascha; Schouler, Catherine; Guabiraba, Rodrigo.
Afiliación
  • Garrido D; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Alber A; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom.
  • Kut E; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Chanteloup NK; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Lion A; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Trotereau A; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Dupont J; INRA, UMR85 Physiologie de la Reproduction et des Comportements, 37380, Nouzilly, France.
  • Tedin K; Institute of Microbiology and Epizootics, Freie Universität Berlin, Germany.
  • Kaspers B; Department of Veterinary Sciences, Institute for Animal Physiology, Ludwig-Maximilians-University, Munich, Germany.
  • Vervelde L; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom.
  • Trapp S; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Schouler C; ISP, INRA, Université de Tours, 37380, Nouzilly, France.
  • Guabiraba R; ISP, INRA, Université de Tours, 37380, Nouzilly, France. Electronic address: rodrigo.guabiraba-brito@inra.fr.
Dev Comp Immunol ; 86: 156-170, 2018 09.
Article en En | MEDLINE | ID: mdl-29729283
Mammalian type I interferons (IFNα/ß) are known to modulate inflammatory processes in addition to their antiviral properties. Indeed, virus-induced type I interferons regulate the mammalian phagocyte immune response to bacteria during superinfections. However, it remains unresolved whether type I IFNs similarly impact the chicken macrophage immune response. We first evidenced that IFNα and IFNß act differently in terms of gene expression stimulation and activation of intracellular signaling pathways in chicken macrophages. Next, we showed that priming of chicken macrophages with IFNα increased bacteria uptake, boosted bacterial-induced ROS/NO production and led to an increased transcriptional expression or production of NOS2/NO, IL1B/IL-1ß and notably IFNB/IFNß. Neutralization of IFNß during bacterial challenge limited IFNα-induced augmentation of the pro-inflammatory response. In conclusion, we demonstrated that type I IFNs differently regulate chicken macrophage functions and drive a pro-inflammatory response to bacterial challenge. These findings shed light on the diverse functions of type I IFNs in chicken macrophages.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Pollos / Interferón beta / Interferón-alfa / Inflamación / Macrófagos Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Pollos / Interferón beta / Interferón-alfa / Inflamación / Macrófagos Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos