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Effect of Silver Nanoparticles on Toxigenic Fusarium spp. and Deoxynivalenol Secretion in Some Grains.
El-Naggar, Medhat A; Alrajhi, Aisha M; Fouda, Moustafa M; Abdelkareem, Eman M; Thabit, Tamer M; Bouqellah, Nahla A.
Afiliación
  • El-Naggar MA; Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
  • Alrajhi AM; Princess Nora University, College of Science, Biology Department, Riyadh, Saudi Arabia.
  • Fouda MM; National Research Centre, Textile Research Division, Giza, Egypt.
  • Abdelkareem EM; Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
  • Thabit TM; Central Agricultural Pesticides Laboratory (CAPL), Agricultural Research Center, Giza, Egypt.
  • Bouqellah NA; Taibah University, College of Science, Biology Department, Al-Madinah Al-Munawarah, Saudi Arabia.
J AOAC Int ; 101(5): 1534-1541, 2018 Sep 01.
Article en En | MEDLINE | ID: mdl-29724266
BACKGROUND: Deoxynivalenol (DON) is one of the most important fungal mycotoxins excreted by different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems. OBJECTIVE: This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains. METHODS: Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA. Tri13 and Tri7 primers were used to evaluate the impact of Ag-NPs on the DNA of tested toxigenic Fusarium isolates. RESULTS: Results revealed that the relative density values (Rd, %) of the isolated Fusarium from barley, wheat, and corn grains were 41.27, 26.47, and 30.76%, respectively. The predominant fungus was F. graminearum and F. culmorum in wheat and barley, respectively. The maximum inhibition diameters used for concentrations were 0.5, 2.8, 3.2, 3.3, and 3.31 mm, respectively. The impact of Ag-NPs on genomic structure was limited. Results demonstrated that Ag-NPs have the ability to reduce the linear growth of Fusarium spp. and eliminate the DON toxin to 34.44, 34.60, and 34.89% at 50, 75, and 100 ppm. CONCLUSIONS: Ag-NPs are considered nontransgenic substances, and their impact on Fusarium DNA under tested concentrations has been neglected. Ag-NPs may work as an alternative to fungicides to reduce fungal growth and eliminate DON mycotoxins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Tricotecenos / Grano Comestible / Nanopartículas del Metal / Fusarium / Antifúngicos Límite: Humans Idioma: En Revista: J AOAC Int Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plata / Tricotecenos / Grano Comestible / Nanopartículas del Metal / Fusarium / Antifúngicos Límite: Humans Idioma: En Revista: J AOAC Int Año: 2018 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido