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Fibroblast Growth Factor 10 Enhances the Developmental Efficiency of Somatic Cell Nuclear Transfer Embryos by Accelerating the Kinetics of Cleavage During In Vitro Maturation.
Son, Yeo-Jin; Lee, Seung-Eun; Park, Yun-Gwi; Jeong, Sang-Gi; Shin, Min-Young; Kim, Eun-Young; Park, Se-Pill.
Afiliación
  • Son YJ; 1 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju, Korea.
  • Lee SE; 2 Stem Cell Research Center, Jeju National University , Jeju, Korea.
  • Park YG; 1 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju, Korea.
  • Jeong SG; 2 Stem Cell Research Center, Jeju National University , Jeju, Korea.
  • Shin MY; 1 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju, Korea.
  • Kim EY; 2 Stem Cell Research Center, Jeju National University , Jeju, Korea.
  • Park SP; 1 Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University , Jeju, Korea.
Cell Reprogram ; 20(3): 196-204, 2018 06.
Article en En | MEDLINE | ID: mdl-29708768
Somatic cell nuclear transfer (SCNT) is required for the generation of transgenic animals as disease models. During the in vitro development of SCNT embryos, the quality of matured oocytes is one of the major factors regulating the developmental potential of embryos. Time-lapse monitoring systems are new tools that assess the developmental capacity of embryos for use in embryo transfer. In this study, we investigated the effect of fibroblast growth factor 10 (FGF 10) on the developmental potential of SCNT embryos. After the in vitro maturation (IVM) of oocytes in IVM medium containing 10 ng/mL FGF 10 (10 F), the polar body extrusion rate was significantly higher than in the control. However, there was no difference in the percentage of fused embryos between the groups. The cleavage and blastocyst formation rates of embryos were significantly increased in the 10 F compared with the control. In addition, the total cell number was higher and the apoptotic index was lower in the 10 F than control at day 7. The messenger RNA (mRNA) expression of genes involved in apoptosis (baculoviral inhibitor of apoptosis repeat containing 5 [BIRC5] and caspase 3 [CASP3]) and development (octamer-binding transcription factor 4 [POU5F1] and sex determining region Y box 2 [SOX2]) increased after 10 F treatment. Furthermore, the kinetics of the first cleavage was faster and the percentage of embryos at cell block was significantly lower in the 10 F group than in the control. These results demonstrate that exposure of oocytes to FGF 10 during IVM promotes developmental competence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Blastocisto / Técnicas de Cultivo de Embriones / Factor 10 de Crecimiento de Fibroblastos / Técnicas de Transferencia Nuclear / Técnicas de Maduración In Vitro de los Oocitos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Reprogram Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Blastocisto / Técnicas de Cultivo de Embriones / Factor 10 de Crecimiento de Fibroblastos / Técnicas de Transferencia Nuclear / Técnicas de Maduración In Vitro de los Oocitos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Reprogram Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos