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Differentiation of Saccharomyces cerevisiae populations from vineyards of the Azores Archipelago: Geography vs Ecology.
Drumonde-Neves, João; Franco-Duarte, Ricardo; Vieira, Eugénia; Mendes, Inês; Lima, Teresa; Schuller, Dorit; Pais, Célia.
Afiliación
  • Drumonde-Neves J; CITAA - Research Center for Agricultural Technology of Azores, University of Azores, Angra do Heroísmo, Portugal; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
  • Franco-Duarte R; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal. Electronic address: ricardofilipeduarte@bio.uminho.pt.
  • Vieira E; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
  • Mendes I; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
  • Lima T; CITAA - Research Center for Agricultural Technology of Azores, University of Azores, Angra do Heroísmo, Portugal.
  • Schuller D; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
  • Pais C; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
Food Microbiol ; 74: 151-162, 2018 Sep.
Article en En | MEDLINE | ID: mdl-29706331
Aiming to elucidate the roles that ecology and geography play in shaping the differentiation of fermentative grape-associated Saccharomyces cerevisiae populations, several locations on six islands of the Azores Archipelago were surveyed. A total of 249 strains were isolated from spontaneous fermentations of grape samples from several varieties of two distinct grapevine species (Vitis vinifera L. and Vitis labrusca L.), in vineyards that are under regular cultivation or in abandoned vineyards. Strains were genetically analyzed using a set of nine microsatellite loci, and also phenotypically characterized using relevant physiological/biotechnological tests. Results showed that genetic divergence among populations of the same island was lower than from populations from different islands. Phenotypic comparison of the populations from each of the islands revealed significant differences between them. Strains isolated from the islands with more intensive viticultural activity - Pico, Terceira and Graciosa - showed higher levels of SO2 tolerance, possibly resulting from selection by human activity. The percentage of strains producing low levels of H2S was higher in S. Jorge (60%). Our findings were supported both by genetic and phenotypic data and provide clear evidence for the prevailing role of the geography over ecology in the differentiation of S. cerevisiae populations in the Azores Archipelago.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Variación Genética / Vitis / Ecología / Geografía Límite: Humans Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Variación Genética / Vitis / Ecología / Geografía Límite: Humans Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido