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PKC delta activation increases neonatal rat retinal cells survival in vitro: Involvement of neurotrophins and M1 muscarinic receptors.
Braga, Luis Eduardo Gomes; Miranda, Renan Lyra; Granja, Marcelo Gomes; Giestal-de-Araujo, Elizabeth; Dos Santos, Aline Araujo.
Afiliación
  • Braga LEG; Programa de Neurociências, Universidade Federal Fluminense, RJ, Brazil.
  • Miranda RL; Programa de Neurociências, Universidade Federal Fluminense, RJ, Brazil.
  • Granja MG; Programa de Neurociências, Universidade Federal Fluminense, RJ, Brazil.
  • Giestal-de-Araujo E; Programa de Neurociências, Universidade Federal Fluminense, RJ, Brazil; Departamento de Neurobiologia, Instituto de Biologia, UFF, Outeiro de São João Batista s/n, Niterói, RJ, CEP: 24020-150, Brazil. Electronic address: egiestal@vm.uff.br.
  • Dos Santos AA; Programa de Neurociências, Universidade Federal Fluminense, RJ, Brazil; Departamento de Fisiologia e Farmacologia, Instituto Biomédico, Universidade Federal Fluminense, Rua Hernani Pires de Mello, 101- São Domingos, Niterói, RJ, CEP: 24210-130, Brazil. Electronic address: alinerabelo@id.uff.br.
Biochem Biophys Res Commun ; 500(4): 917-923, 2018 06 12.
Article en En | MEDLINE | ID: mdl-29705702
Protein kinase C (PKC) is a family of serine/threonine kinases related to several phenomena as cell proliferation, differentiation and survival. Our previous data demonstrated that treatment of axotomized neonatal rat retinal cell cultures for 48 h with phorbol 12-myristate 13-acetate (PMA), a PKC activator, increases retinal ganglion cells (RGCs) survival. Moreover, this treatment decreases M1 receptors (M1R) and modulates BDNF levels. The aim of this work was to assess the possible involvement of neurotrophins BDNF and NGF in the modulation of M1R levels induced by PKC activation, and its involvement on RGCs survival. Our results show that PMA (50 ng/mL) treatment, via PKC delta activation, modulates NGF, BDNF and M1R levels. BDNF and NGF mediate the decrease of M1R levels induced by PMA treatment. M1R activation is essential to PMA neuroprotective effect on RGCs as telenzepine (M1R selective antagonist) abolished it. Based on our results we suggest that PKC delta activation modulates neurotrophins levels by a signaling pathway that involves M1R activation and ultimately leading to an increase in RGCs survival in vitro.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Ganglionares de la Retina / Acetato de Tetradecanoilforbol / Agonistas Muscarínicos / Factor Neurotrófico Derivado del Encéfalo / Factor de Crecimiento Nervioso / Receptor Muscarínico M1 / Proteína Quinasa C-delta Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Ganglionares de la Retina / Acetato de Tetradecanoilforbol / Agonistas Muscarínicos / Factor Neurotrófico Derivado del Encéfalo / Factor de Crecimiento Nervioso / Receptor Muscarínico M1 / Proteína Quinasa C-delta Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos