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Sperm chromatin alterations in fertile and subfertile bulls.
Souza, Elisson Terêncio; Silva, Cláudio Vieira; Travençolo, Bruno Augusto Nassif; Alves, Benner Geraldo; Beletti, Marcelo Emílio.
Afiliación
  • Souza ET; Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil.
  • Silva CV; Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil.
  • Travençolo BAN; Faculty of Computer Science, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil.
  • Alves BG; Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil.
  • Beletti ME; Laboratory of Biology of Reproduction, Institute of Biomedical Sciences, Federal University of Uberlândia, CEP 38400-902, Uberlândia, MG, Brazil. Electronic address: mebeletti@ufu.br.
Reprod Biol ; 18(2): 177-181, 2018 Jun.
Article en En | MEDLINE | ID: mdl-29705068
Alterations in sperm chromatin have been related with subfertility in several mammals. In this study, chromatin alteration types (Base, Basal half, Central axis, Dispersed, and Whole) were assessed by toluidine blue (TB) staining, 6-diamidino-2-fenilindole (DAPI) and anti-protamine 1 antibody (anti-PR1) labeling in sperm samples of fertile and subfertile bulls. Semen samples were obtained from bulls kept in Artificial Insemination Center (fertile bulls) or from bulls subjected to scrotal insulation (subfertile bulls). The percentage of chromatin alterations identified by TB was similar (P > 0.05) in semen samples of fertile and subfertile bulls. In contrast, a greater (P < 0.01) chromatin decondensation and heterogeneity were recorded in semen samples of subfertile bulls. In DAPI and anti-PR1 methods, the subfertile bulls samples had a higher (P < 0.05) percentage of alteration in the base as well as overall chromatin alterations (P < 0.05). Moreover, the chromatin alterations recorded with TB, DAPI, and anti-PR1 were compared in semen samples of fertile and subfertile bulls. In fertile bulls, the overall chromatin alterations were similar (P > 0.05) among the methods In contrast, semen samples of subfertile bulls had a higher (P < 0.05) percentage of overall chromatin alterations when labeled with DAPI. In conclusion, our findings shown that all dye tested had specific sperm stainability and can be feasible to monitor subfertility condition in bulls. Also, different chromatin alteration types in sperm samples of fertile and suberftile bulls were recorded.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Motilidad Espermática / Espermatozoides / Cromatina / Fertilidad / Infertilidad Masculina Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Reprod Biol Asunto de la revista: MEDICINA REPRODUTIVA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Polonia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Motilidad Espermática / Espermatozoides / Cromatina / Fertilidad / Infertilidad Masculina Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Reprod Biol Asunto de la revista: MEDICINA REPRODUTIVA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Polonia