Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics.
Plant Physiol
; 177(2): 476-489, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29703867
Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reguladores del Crecimiento de las Plantas
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Arabidopsis
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Espectrometría de Masas en Tándem
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Metabolómica
Idioma:
En
Revista:
Plant Physiol
Año:
2018
Tipo del documento:
Article
País de afiliación:
República Checa
Pais de publicación:
Estados Unidos