Your browser doesn't support javascript.
loading
In vitro photodynamic activity of lipid vesicles with zinc phthalocyanine derivative against Enterococcus faecalis.
Sobotta, Lukasz; Dlugaszewska, Jolanta; Kasprzycki, Piotr; Lijewski, Sebastian; Teubert, Anna; Mielcarek, Jadwiga; Gdaniec, Maria; Goslinski, Tomasz; Fita, Piotr; Tykarska, Ewa.
Afiliación
  • Sobotta L; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, Poznan 60-780, Poland. Electronic address: lsobotta@ump.edu.pl.
  • Dlugaszewska J; Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, Poznan 60-780, Poland.
  • Kasprzycki P; Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, Warsaw 02-093, Poland.
  • Lijewski S; Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Grunwaldzka 6, Poznan 60-780, Poland.
  • Teubert A; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Z. Noskowskiego 12/14, Poznan, Poland.
  • Mielcarek J; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, Poznan 60-780, Poland.
  • Gdaniec M; Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, Poznan 61-614, Poland.
  • Goslinski T; Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Grunwaldzka 6, Poznan 60-780, Poland.
  • Fita P; Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, Warsaw 02-093, Poland.
  • Tykarska E; Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Grunwaldzka 6, Poznan 60-780, Poland.
J Photochem Photobiol B ; 183: 111-118, 2018 Jun.
Article en En | MEDLINE | ID: mdl-29702340
Zinc(II) phthalocyanine bearing eight non-peripheral 2-propoxy substituents was subjected to physicochemical study and, after incorporation in lipid vesicles, assessed as a potential photosensitizer for antibacterial photodynamic therapy. The phthalocyanine derivative obtained in the macrocyclization reaction was characterized by MS and NMR techniques. Moreover, its chemical purity was confirmed by HPLC analysis. X-ray structural analysis revealed that overcrowding of the phthalocyanine derivative leads to a strong out-of-plane distortion of the π-system of the macrocycle core. In the UV-Vis absorption spectra of zinc(II) phthalocyanine two characteristic bands were found: the Soret (300-450 nm) and the Q band (600-800 nm). Photophysical properties of mono- and diprotonated forms of phthalocyanine derivative were studied with time-resolved fluorescence spectroscopy. Its tri- and tetraprotonated forms could not be obtained, because compound decomposes in higher acid concentrations. The presented zinc(II) phthalocyanine showed values of singlet oxygen generation ΦΔ = 0.18 and 0.16, the quantum yield of the photodecomposition ΦP = 3.06∙10-4 and 1.23∙10-5 and the quantum yield of fluorescence ΦFL = 0.005 and 0.004, designated in DMF and DMSO, respectively. For biological studies, phthalocyanine has been incorporated into modified liposome vesicles containing ethanol. In vitro bacteria photoinactivation study revealed no activity against Escherichia coli and 5.7 log reduction of the Enterococcus faecalis growth.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos Organometálicos / Enterococcus faecalis / Fármacos Fotosensibilizantes / Indoles / Liposomas Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos Organometálicos / Enterococcus faecalis / Fármacos Fotosensibilizantes / Indoles / Liposomas Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Suiza