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Tissue distribution, shedding and environmental detection of infectious bursal disease virus genome following infection of meat chickens at two ages.
Jayasundara, Jmkgk; Walkden-Brown, S W; Islam, Afmf; Katz, M E; Renz, K G.
Afiliación
  • Jayasundara J; Animal Science, School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia.
  • Walkden-Brown SW; Animal Science, School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia.
  • Islam A; Animal Science, School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia.
  • Katz ME; Molecular and Cellular Biology, School of Science and Technology, University of New England, Armidale, NSW, Australia.
  • Renz KG; Animal Science, School of Environmental and Rural Science, University of New England, Armidale, New South Wales 2351, Australia.
Aust Vet J ; 96(5): 167-175, 2018 May.
Article en En | MEDLINE | ID: mdl-29691850
OBJECTIVE: To compare the effects of infectious bursal disease virus (IBDV) infection of commercial meat chickens at 0 and 16 days old (d.o.) and determine if IBDV vRNA is quantifiable in litter and dust samples. METHODS: Ross meat chickens (n = 60) were orally infected or not with IBDV at 0 or 16 d.o. Blood and faecal samples were collected longitudinally to 28 days post infection (dpi) from six chickens and tissues collected weekly from three euthanased chickens. Relative bursal weight was recorded postmortem. IBDV antibody titres in sera were measured using ELISA and VCN was determined in tissues, faeces, litter and dust using qRT-PCR. RESULTS: Chickens infected at 16 d.o. had earlier and more severe bursal atrophy, earlier and higher IBDV vRNA load in lymphoid organs and an earlier and greater antibody response to infection than those infected at 0 d.o. Faecal shedding of IBDV between 2 and 6 dpi was observed in both groups followed by cessation with the 0 d.o. group and re-initiation of shedding at 28 dpi. IBDV was readily detected and quantified in litter and dust samples. CONCLUSIONS: The presence of significant maternal antibody (MAb) titres in 0 d.o. chickens provided protection against IBDV replication and bursal atrophy at 7 and 14 days post infection. The reduced titres of MAb present at 16 d.o. did not prevent rapid IBDV replication and early marked bursal atrophy. The observed resistance of 0 d.o. chickens is likely to be a combination of MAb inhibition of IBDV and true age resistance of neonatal chicks. Measurement of IBDV in litter and dust may have research or diagnostic application.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Pollos / Infecciones por Birnaviridae / Virus de la Enfermedad Infecciosa de la Bolsa Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: Aust Vet J Año: 2018 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Pollos / Infecciones por Birnaviridae / Virus de la Enfermedad Infecciosa de la Bolsa Tipo de estudio: Diagnostic_studies Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: Aust Vet J Año: 2018 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido