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Application of MALDI-TOF MS for the subtyping of Arcobacter butzleri strains and comparison with their MLST and PFGE types.
Giacometti, Federica; Piva, Silvia; Vranckx, Katleen; De Bruyne, Katrien; Drigo, Ilenia; Lucchi, Alex; Manfreda, Gerardo; Serraino, Andrea.
Afiliación
  • Giacometti F; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Italy. Electronic address: federica.giacometti3@unibo.it.
  • Piva S; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Italy. Electronic address: silvia.piva@unibo.it.
  • Vranckx K; Applied Maths NV, Sint-Martens-Latem, Belgium. Electronic address: Katleen_Vranckx@applied-maths.com.
  • De Bruyne K; Applied Maths NV, Sint-Martens-Latem, Belgium. Electronic address: katrien_debruyne@applied-maths.com.
  • Drigo I; Istituto Zooprofilattico Sperimentale delle Venezie, Villorba, Treviso, Italy. Electronic address: idrigo@izsvenezie.it.
  • Lucchi A; Department of Agricultural and Food Sciences, University of Bologna, Ozzano dell'Emilia, Italy. Electronic address: alex.lucchi3@unibo.it.
  • Manfreda G; Department of Agricultural and Food Sciences, University of Bologna, Ozzano dell'Emilia, Italy. Electronic address: gerardo.manfreda@unibo.it.
  • Serraino A; Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Italy. Electronic address: andrea.serraino@unibo.it.
Int J Food Microbiol ; 277: 50-57, 2018 Jul 20.
Article en En | MEDLINE | ID: mdl-29684765
For the first time, this study evaluated the use of MALDI-TOF as a typing tool for Arcobacter butzleri. A total of 104 A. butzleri strains isolated from different sources in an artisanal dairy plant in Italy were identified and typed using MALDI-TOF and compared with their multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) profiles found in previous studies. MALDI-TOF correctly identified all the isolates to species level. No clearly delineated clusters appeared on dendrograms based on either the complete spectra or the significant peaks, but nine clusters were defined using the cophenetic correlation. Interestingly, MALDI-TOF proved able to discriminate A. butzleri strains below species level, confirming its potential use for epidemiological surveys. As expected, the comparative analysis with PFGE and MLST showed that the discriminatory index was lower for MALDI-TOF but roughly comparable to sequence types and pulsotypes. MALDI-TOF appears to be a relatively low cost answer to the urgent need for more rapid, less expensive typing tools suitable for source attribution studies, readily allowing multiple typing methods to be combined. This study provides insights into MALDI-TOF as potential epidemiological tool. Its application in healthcare surveillance systems awaits further exploration to encourage interaction and convergence studies between primary care in humans and animal and food veterinary authorities as part of the One Health concept.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroforesis en Gel de Campo Pulsado / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Arcobacter / Leche / Tipificación de Secuencias Multilocus Límite: Animals / Humans País/Región como asunto: Europa Idioma: En Revista: Int J Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroforesis en Gel de Campo Pulsado / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Arcobacter / Leche / Tipificación de Secuencias Multilocus Límite: Animals / Humans País/Región como asunto: Europa Idioma: En Revista: Int J Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos