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Primary transcriptome analysis reveals importance of IS elements for the shaping of the transcriptional landscape of Bordetella pertussis.
Amman, Fabian; D'Halluin, Alexandre; Antoine, Rudy; Huot, Ludovic; Bibova, Ilona; Keidel, Kristina; Slupek, Stéphanie; Bouquet, Peggy; Coutte, Loïc; Caboche, Ségolène; Locht, Camille; Vecerek, Branislav; Hot, David.
Afiliación
  • Amman F; a University of Vienna, Theoretical Biochemistry Group, Institute for Theoretical Chemistry , Vienna , Austria.
  • D'Halluin A; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Antoine R; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Huot L; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Bibova I; c Institute of Microbiology of the ASCR; Laboratory of post-transcriptional control of gene expression , Prague , Czech Republic.
  • Keidel K; c Institute of Microbiology of the ASCR; Laboratory of post-transcriptional control of gene expression , Prague , Czech Republic.
  • Slupek S; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Bouquet P; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Coutte L; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Caboche S; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Locht C; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
  • Vecerek B; c Institute of Microbiology of the ASCR; Laboratory of post-transcriptional control of gene expression , Prague , Czech Republic.
  • Hot D; b Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR8204 - CIIL - Center for Infection and Immunity of Lille , Lille , France.
RNA Biol ; 15(7): 967-975, 2018.
Article en En | MEDLINE | ID: mdl-29683387
Bordetella pertussis is the causative agent of whooping cough, a respiratory disease still considered as a major public health threat and for which recent re-emergence has been observed. Constant reshuffling of Bordetella pertussis genome organization was observed during evolution. These rearrangements are essentially mediated by Insertion Sequences (IS), a mobile genetic elements present in more than 230 copies in the genome, which are supposed to be one of the driving forces enabling the pathogen to escape from vaccine-induced immunity. Here we use high-throughput sequencing approaches (RNA-seq and differential RNA-seq), to decipher Bordetella pertussis transcriptome characteristics and to evaluate the impact of IS elements on transcriptome architecture. Transcriptional organization was determined by identification of transcription start sites and revealed also a large variety of non-coding RNAs including sRNAs, leaderless mRNAs or long 3' and 5'UTR including seven riboswitches. Unusual topological organizations, such as overlapping 5'- or 3'-extremities between oppositely orientated mRNA were also unveiled. The pivotal role of IS elements in the transcriptome architecture and their effect on the transcription of neighboring genes was examined. This effect is mediated by the introduction of IS harbored promoters or by emergence of hybrid promoters. This study revealed that in addition to their impact on genome rearrangements, most of the IS also impact on the expression of their flanking genes. Furthermore, the transcripts produced by IS are strain-specific due to the strain to strain variation in IS copy number and genomic context.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Bordetella pertussis / ARN Bacteriano / Elementos Transponibles de ADN / Perfilación de la Expresión Génica Tipo de estudio: Prognostic_studies Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcripción Genética / Bordetella pertussis / ARN Bacteriano / Elementos Transponibles de ADN / Perfilación de la Expresión Génica Tipo de estudio: Prognostic_studies Idioma: En Revista: RNA Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos