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Graft Protection Against Cold Ischemia Preservation: An Institute George Lopez 1 and Histidine-tryptophan-ketoglutarate Solution Appraisal.
Panisello-Rosello, A; Castro-Benítez, C; Lopez, A; Balloji, S; Folch-Puy, E; Adam, R; Roselló-Catafau, J.
Afiliación
  • Panisello-Rosello A; Institut d'Investigacions Biomèdiques de Barcelona (IIBB), CSIC-IDIBAPS, Catalonia, Barcelona, Spain.
  • Castro-Benítez C; Centre Hepato-Biliare, AP-P-HP Hôpital Paul Brousse, Inserm U776, Univesité Paris-Sud, Villejuif, Paris, France.
  • Lopez A; Centre Hepato-Biliare, AP-P-HP Hôpital Paul Brousse, Inserm U776, Univesité Paris-Sud, Villejuif, Paris, France.
  • Balloji S; Institut d'Investigacions Biomèdiques de Barcelona (IIBB), CSIC-IDIBAPS, Catalonia, Barcelona, Spain.
  • Folch-Puy E; Institut d'Investigacions Biomèdiques de Barcelona (IIBB), CSIC-IDIBAPS, Catalonia, Barcelona, Spain.
  • Adam R; Centre Hepato-Biliare, AP-P-HP Hôpital Paul Brousse, Inserm U776, Univesité Paris-Sud, Villejuif, Paris, France.
  • Roselló-Catafau J; Institut d'Investigacions Biomèdiques de Barcelona (IIBB), CSIC-IDIBAPS, Catalonia, Barcelona, Spain. Electronic address: jrcbam@iibb.csic.es.
Transplant Proc ; 50(3): 714-718, 2018 Apr.
Article en En | MEDLINE | ID: mdl-29661422
Cold storage of organs in preservation solutions, such as Institute George Lopez 1 (IGL-1) or histidine-tryptophan-ketoglutarate (HTK), is a mandatory step for organ transplantation. This preservation leads to an ischemic injury that affects the outcome of the organ. This article studies the liver graft eluate after organ recovery using IGL-1 or HTK solutions. We explore the influence of the volume used for washing out the liver and the consequences in the graft preservation when both solutions are used. Livers were washed out with different volumes of HTK and IGL-1 according to manufacturers' instructions and then preserved in both solutions for 24 hours at 4°C. Tissue and eluates were collected for subsequent analyses. We measured transaminases (aspartate aminotransferase and alanine aminotransferase), histology by hematoxylin/eosin staining, and red blood cell and hemoglobin counts, respectively. After washing out and cold storage, the IGL-1 processed livers showed better preservation than those with HTK solution; however, in this latter case, an important accumulation of erythrocytes was found when compared to IGL-1. These data were consistent with the higher hemoglobin and red blood cell counts observed for IGL-1 eluates after 24 hours. The volume used for washing out the organ depends on the composition and properties of the organ preservation solutions (ie, IGL-1 and HTK); this is an important factor for the graft cold preservation. The total volume used for washing out the graft should be considered because it has a direct impact on the total cost for clinical transplantations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Soluciones Preservantes de Órganos / Isquemia Fría Límite: Animals / Humans / Male Idioma: En Revista: Transplant Proc Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Soluciones Preservantes de Órganos / Isquemia Fría Límite: Animals / Humans / Male Idioma: En Revista: Transplant Proc Año: 2018 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos