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Cystoid edema, neovascularization and inflammatory processes in the murine Norrin-deficient retina.
Beck, Susanne C; Karlstetter, Marcus; Garcia Garrido, Marina; Feng, Yuxi; Dannhausen, Katharina; Mühlfriedel, Regine; Sothilingam, Vithiyanjali; Seebauer, Britta; Berger, Wolfgang; Hammes, Hans-Peter; Seeliger, Mathias W; Langmann, Thomas.
Afiliación
  • Beck SC; Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, Centre for Ophthalmology, Tuebingen, Germany. su.beck@uni-tuebingen.de.
  • Karlstetter M; Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, D-50931, Cologne, Germany.
  • Garcia Garrido M; Bayer AG, Wuppertal, Germany.
  • Feng Y; Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, Centre for Ophthalmology, Tuebingen, Germany.
  • Dannhausen K; Institute of Experimental and Clinical Pharmacology and Toxicology, Medical Faculty Mannheim, University of Heidelberg, D-68169, Mannheim, Germany.
  • Mühlfriedel R; Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, D-50931, Cologne, Germany.
  • Sothilingam V; Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, Centre for Ophthalmology, Tuebingen, Germany.
  • Seebauer B; Division of Ocular Neurodegeneration, Institute for Ophthalmic Research, Centre for Ophthalmology, Tuebingen, Germany.
  • Berger W; Institute of Medical Molecular Genetics, University of Zurich, Zurich, Switzerland.
  • Hammes HP; Institute of Medical Molecular Genetics, University of Zurich, Zurich, Switzerland.
  • Seeliger MW; Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland.
  • Langmann T; Neuroscience Center Zurich (ZNZ), University and ETH Zurich, Zurich, Switzerland.
Sci Rep ; 8(1): 5970, 2018 04 13.
Article en En | MEDLINE | ID: mdl-29654250
Mutations in the Norrin (NDP) gene cause severe developmental blood vessel defects in the retina leading to congenital blindness. In the retina of Ndph-knockout mice only the superficial capillary network develops. Here, a detailed characterization of this mouse model at late stages of the disease using in vivo retinal imaging revealed cystoid structures that closely resemble the ovoid cysts in the inner nuclear layer of the human retina with cystoid macular edema (CME). In human CME an involvement of Müller glia cells is hypothesized. In Ndph-knockout retinae we could demonstrate that activated Müller cells were located around and within these cystoid spaces. In addition, we observed extensive activation of retinal microglia and development of neovascularization. Furthermore, ex vivo analyses detected extravasation of monocytic cells suggesting a breakdown of the blood retina barrier. Thus, we could demonstrate that also in the developmental retinal vascular pathology present in the Ndph-knockout mouse inflammatory processes are active and may contribute to further retinal degeneration. This observation delivers a new perspective for curative treatments of retinal vasculopathies. Modulation of inflammatory responses might reduce the symptoms and improve visual acuity in these diseases.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Edema Macular / Proteínas del Ojo / Inflamación / Neovascularización Patológica / Proteínas del Tejido Nervioso Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Edema Macular / Proteínas del Ojo / Inflamación / Neovascularización Patológica / Proteínas del Tejido Nervioso Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido