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Gα-cAMP/PKA pathway positively regulates pigmentation, chaetoglobosin A biosynthesis and sexual development in Chaetomium globosum.
Hu, Yang; Hao, Xiaoran; Chen, Longfei; Akhberdi, Oren; Yu, Xi; Liu, Yanjie; Zhu, Xudong.
Afiliación
  • Hu Y; Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
  • Hao X; National Experimental Teaching Demonstrating Center, School of Life Sciences, Beijing Normal University, Beijing, China.
  • Chen L; Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
  • Akhberdi O; Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
  • Yu X; Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, China.
  • Liu Y; Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, School of Life Sciences, Beijing Normal University, Beijing, China.
  • Zhu X; Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, School of Life Sciences, Beijing Normal University, Beijing, China.
PLoS One ; 13(4): e0195553, 2018.
Article en En | MEDLINE | ID: mdl-29652900
Sensing the environmental signals, the canonical Gα-cAMP/PKA pathway modulates mycelial growth and development, and negatively regulates some secondary metabolism in filamentous fungi, e.g. aflatoxin in Aspergillus nidulans. Here we report the characterization of this signaling pathway in Chaetomium globosum, a widely spread fungus known for synthesizing abundant secondary metabolites, e.g. chaetoglobosin A (ChA). RNAi-mediated knockdown of a putative Gα-encoding gene gna-1, led to plural changes in phenotype, e.g. albino mycelium, significant restriction on perithecium development and decreased production of ChA. RNA-seq profiling and qRT-PCR verified significantly fall in expression of corresponding genes, e.g. pks-1 and CgcheA. These defects could be restored by simultaneous knock-down of the pkaR gene encoding a regulatory subunit of cAMP-dependent protein kinase A (PKA), suggesting that pkaR had a negative effect on the above mentioned traits. Confirmatively, the intracellular level of cAMP in wild-type strain was about 3.4-fold to that in gna-1 silenced mutant pG14, and addition of a cAMP analog, 8-Br-cAMP, restored the same defects, e.g., the expression of CgcheA. Furthermore, the intracellular cAMP in gna-1 and pkaR double silenced mutant was approaching the normal level. The following activity inhibition experiment proved that the expression of CgcheA was indeed regulated by PKA. Down-regulation of LaeA/VeA/SptJ expression in gna-1 mutant was also observed, implying that Gα signaling may crosstalk to other regulatory pathways. Taken together, this study proposes that the heterotrimeric Gα protein-cAMP/PKA signaling pathway positively mediates the sexual development, melanin biosynthesis, and secondary metabolism in C. globosum.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pigmentación / Chaetomium / Proteínas Quinasas Dependientes de AMP Cíclico / AMP Cíclico / Alcaloides Indólicos / Subunidades alfa de la Proteína de Unión al GTP Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pigmentación / Chaetomium / Proteínas Quinasas Dependientes de AMP Cíclico / AMP Cíclico / Alcaloides Indólicos / Subunidades alfa de la Proteína de Unión al GTP Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos