Within-subject biological variation of activated partial thromboplastin time, prothrombin time, fibrinogen, factor VIII and von Willebrand factor in pregnant women.

Kristoffersen, Ann Helen; Petersen, Per Hyltoft; Bjørge, Line; Røraas, Thomas; Sandberg, Sverre

Kristoffersen, Ann Helen; Petersen, Per Hyltoft; Bjørge, Line; Røraas, Thomas; Sandberg, Sverre.

Afiliación

Kristoffersen AH; Laboratory of Clinical Biochemistry, Helse Bergen HF, Haukeland University Hospital, PB 1400, Bergen, Norway.
Petersen PH; Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
Bjørge L; Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
Røraas T; Centre for Cancer Biomarkers CCBIO, Department of Clinical Science, University of Bergen, Bergen, Norway.
Sandberg S; Department of Gynecology and Obstetrics, Haukeland University Hospital, Bergen, Norway.

Clin Chem Lab Med ; 56(8): 1297-1308, 2018 07 26.

Article en En | MEDLINE | ID: mdl-29648994

RESUMEN

BACKGROUND: During pregnancy, interpretation of results from coagulation parameters can be difficult as the physiological changes that occur may affect the biochemical parameters. The aim of this study was to describe the normal course of five coagulation parameters in healthy pregnancies, and to estimate the within-subject biological variation (CVI). METHODS: Blood samples were obtained every 4th week during pregnancy and three samples after delivery in 20 healthy women and every 4th week during a 40-week period in 19 healthy non-pregnant women. Activated partial thromboplastin time (APTT), prothrombin time (PT), PT International Normalized Ratio (INR), fibrinogen, factor VIII clot (FVIII:C) and von Willebrand factor antigen (vWF:Ag) were analyzed. The physiological changes during pregnancy were compensated by transformation into multiples of the median (MoM) and it is natural logarithm (lnMoM) in order to establish a kind of steady state, and CVI was calculated from the standard deviation. RESULTS: During pregnancy, APTT, PT and INR remained unchanged or decreased, depending upon the reagent used, while fibrinogen, FVIII:C and vWF:Ag increased gradually until delivery. The CVI in pregnancy were 2.2 and 3.0% for APTT, 2.3 and 2.6% for PT, 2.2 and 2.3% for INR, 7.2% for fibrinogen, 12.2% for FVIII:C and 11.3% for vWF:Ag, and corresponded with the CVI in non-pregnant women. CONCLUSIONS: Transformation of coagulation parameters in healthy pregnancies to MoM is a tool to establish a kind of steady state. Although there is a physiological change in these coagulation parameters during pregnancy, the CVI after lnMoM transformation was comparable with the CVI of non-pregnant women.

Asunto(s)

Variación Biológica Individual; Factor VIII/metabolismo; Fibrinógeno/metabolismo; Tiempo de Protrombina; Factor de von Willebrand/metabolismo; Factor VIII/análisis; Femenino; Fibrinógeno/análisis; Humanos; Tiempo de Tromboplastina Parcial; Embarazo; Factor de von Willebrand/análisis

Palabras clave

biological variation; blood coagulation tests; haemostasis; multiples of median; pregnancy

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tiempo de Protrombina / Factor VIII / Fibrinógeno / Factor de von Willebrand / Variación Biológica Individual Límite: Female / Humans / Pregnancy Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Alemania

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google