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Improving Lentiviral Transduction of CD34+ Hematopoietic Stem and Progenitor Cells.
Hauber, Ilona; Beschorner, Niklas; Schrödel, Silke; Chemnitz, Jan; Kröger, Nicolaus; Hauber, Joachim; Thirion, Christian.
Afiliación
  • Hauber I; 1 Heinrich Pette Institute - Leibniz Institute for Experimental Virology , Hamburg, Germany.
  • Beschorner N; 1 Heinrich Pette Institute - Leibniz Institute for Experimental Virology , Hamburg, Germany.
  • Schrödel S; 2 German Center for Infection Research (DZIF) , Partner Site Hamburg, Germany.
  • Chemnitz J; 3 SIRION Biotech GmbH, Planegg-Martinsried , Germany.
  • Kröger N; 1 Heinrich Pette Institute - Leibniz Institute for Experimental Virology , Hamburg, Germany.
  • Hauber J; 4 Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf , Hamburg, Germany .
  • Thirion C; 1 Heinrich Pette Institute - Leibniz Institute for Experimental Virology , Hamburg, Germany.
Hum Gene Ther Methods ; 29(2): 104-113, 2018 04.
Article en En | MEDLINE | ID: mdl-29631437
The delivery of therapeutic genes for treatment of inherited or infectious diseases frequently requires lentiviral transduction of CD34+ hematopoietic stem and progenitor cells (HSC). Optimized transduction protocols with a therapeutic goal aim to maximize the number of transduction-positive cells while limiting the vector copy number that reach each individual cell. Importantly, the transduced HSC should maintain their "stem-like" properties. Here, we analyzed LentiBOOST™ reagent, a membrane-sealing poloxamer, with respect to enhancing lentiviral transduction of CD34+ peripheral blood stem cells. We demonstrate that inclusion of LentiBOOST™ in a standard HSC transduction protocol yields high transduction efficiencies while preserving the ability of the transduced HSC to differentiate into various hematopoietic lineages. Thus, LentiBOOST™ reagent can significantly improve lentiviral CD34+ HSC transduction protocols with the potential to improve production of gene-modified cell products.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción Genética / Células Madre Hematopoyéticas / Linfocitos T / VIH-1 / Reacción en Cadena en Tiempo Real de la Polimerasa / Vectores Genéticos Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Hum Gene Ther Methods Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción Genética / Células Madre Hematopoyéticas / Linfocitos T / VIH-1 / Reacción en Cadena en Tiempo Real de la Polimerasa / Vectores Genéticos Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Hum Gene Ther Methods Año: 2018 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos