Structure characterization and antitumor activity of the extracellular polysaccharide from the marine fungus Hansfordia sinuosae.

Li, Hongyan; Cao, Kewei; Cong, Peixu; Liu, Yanjun; Cui, Huanhuan; Xue, Changhu

Li, Hongyan; Cao, Kewei; Cong, Peixu; Liu, Yanjun; Cui, Huanhuan; Xue, Changhu.

Afiliación

Li H; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: lihongyan1447@163.com.
Cao K; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Cong P; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Liu Y; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Cui H; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Xue C; College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: xuechanghuouc@163.com.

Carbohydr Polym ; 190: 87-94, 2018 Jun 15.

Article en En | MEDLINE | ID: mdl-29628263

RESUMEN

A neutral water soluble polysaccharide (HPA) was isolated from the marine fungus Hansfordia sinuosae. Monosaccharide composition analysis indicated that HPA was mainly composed of mannose with minor amounts of galactose and glucose. The molecular weight of HPA was approximately 22.5â¯kDa as analyzed by HPGPC. Structure analysis of HPA with methylation and 1D, 2D NMR indicated that HPA was composed of [α-d-Manp(1â], [â2)-α-d-Manp(1â], [â3)-α-d-Manp(1â] and [â2,6)-α-d-Manp(1â] with [α-d-Manp(1â] linked to C-6 position of [â2,6)-α-d-Manp(1â]. The antitumor effect of HPA was evaluated in vitro. HPA showed remarkable inhibitory effect on human cervical carcinoma HeLa cells and human breast carcinoma MCF-7 cells. When cells were incubated with HPA at 400â¯µg/mL for 48â¯h, the inhibition rate on HeLa and MCF-7 cells was 79.5% and 73.8%, respectively. Furthermore, for HeLa cells, HPA could increase intracellular ROS levels, induce cells apoptosis, decrease mitochondrial membrane potential, and elevate the expression of caspase-3. The results suggested that HPA could be explored as a potential antitumor agent.

Asunto(s)

Antineoplásicos/química; Antineoplásicos/farmacología; Ascomicetos/citología; Espacio Extracelular/química; Polisacáridos Fúngicos/química; Polisacáridos Fúngicos/farmacología; Apoptosis/efectos de los fármacos; Caspasa 3/metabolismo; Proliferación Celular/efectos de los fármacos; Regulación Neoplásica de la Expresión Génica/efectos de los fármacos; Células HeLa; Humanos; Espacio Intracelular/efectos de los fármacos; Espacio Intracelular/metabolismo; Células MCF-7; Potencial de la Membrana Mitocondrial/efectos de los fármacos; Metilación/efectos de los fármacos; Monosacáridos/análisis; Especies Reactivas de Oxígeno/metabolismo

Palabras clave

1-Phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051); Acetic anhydride (PubChem CID: 7918); Antitumor activity; Apoptosis; Dichloromethane (PubChem CID: 6344); Dimethyl sulfoxide (PubChem CID: 679); Extracellular polysaccharide; Hansfordia sinuosae; Iodomethane (PubChem CID: 6328); Phenol (PubChem CID: 996); Pullulan (PubChem CID: 92024139); Pyridine (PubChem CID: 1049); Sodium chloride (PubChem CID:5234); Sodium sulfate (PubChem CID: 24436); Structure; Trifluoroacetic acid (PubChem CID: 6422); d-Mannose (PubChem CID: 18950)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Espacio Extracelular / Polisacáridos Fúngicos / Antineoplásicos Límite: Humans Idioma: En Revista: Carbohydr Polym Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido

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