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Phyllobacterium zundukense sp. nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers.
Safronova, Vera I; Sazanova, Anna L; Kuznetsova, Irina G; Belimov, Andrey A; Andronov, Evgeny E; Chirak, Elizaveta R; Popova, Janna P; Verkhozina, Alla V; Willems, Anne; Tikhonovich, Igor A.
Afiliación
  • Safronova VI; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Sazanova AL; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Kuznetsova IG; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Belimov AA; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Andronov EE; Kazan Institute of Biochemistry and Biophysics of the Russian Academy of Sciences (KIBB of RAS), Lobachevsky Str. 2/31, 420111 Kazan, Russian Federation.
  • Chirak ER; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Popova JP; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Verkhozina AV; All-Russia Research Institute for Agricultural Microbiology (ARRIAM), 196608, St.-Petersburg, Sh. Podbelskogo 3, Russian Federation.
  • Willems A; Siberian Institute of Plant Physiology and Biochemistry (SIPPB), Lermontova Str. 132, 664033 Irkutsk, Russian Federation.
  • Tikhonovich IA; Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Int J Syst Evol Microbiol ; 68(5): 1644-1651, 2018 May.
Article en En | MEDLINE | ID: mdl-29620492
Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains Phyllobacterium sophorae LMG 27899T, Phyllobacterium brassicacearum LMG 22836T, Phyllobacterium endophyticum LMG 26470T and Phyllobacterium bourgognense LMG 22837T while similarity levels between the isolates and the most closely related strain P. endophyticum LMG 26470T were 98.8-99.5 %. The recA and glnII genes of the isolates showed highest sequence similarities with P. sophorae LMG 27899T (95.4 and 89.5 %, respectively) and P. brassicacearum LMG 22836T (91.4 and 85.1 %, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48T and P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T ranged from 79.23 % for P. endophyticum LMG 26470T to 85.74 % for P. sophorae LMG 27899T. The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48T, although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48T=LMG 30371T=RCAM 03910T), is proposed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Oxytropis / Nódulos de las Raíces de las Plantas / Phyllobacteriaceae País/Región como asunto: Asia / Europa Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Oxytropis / Nódulos de las Raíces de las Plantas / Phyllobacteriaceae País/Región como asunto: Asia / Europa Idioma: En Revista: Int J Syst Evol Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido