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Ploidy Variation in Kluyveromyces marxianus Separates Dairy and Non-dairy Isolates.
Ortiz-Merino, Raúl A; Varela, Javier A; Coughlan, Aisling Y; Hoshida, Hisashi; da Silveira, Wendel B; Wilde, Caroline; Kuijpers, Niels G A; Geertman, Jan-Maarten; Wolfe, Kenneth H; Morrissey, John P.
Afiliación
  • Ortiz-Merino RA; School of Medicine, UCD Conway Institute, University College Dublin, Dublin, Ireland.
  • Varela JA; School of Microbiology, Centre for Synthetic Biology and Biotechnology, Environmental Research Institute, APC Microbiome Institute, University College Cork, Cork, Ireland.
  • Coughlan AY; School of Medicine, UCD Conway Institute, University College Dublin, Dublin, Ireland.
  • Hoshida H; Department of Applied Chemistry, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi, Japan.
  • da Silveira WB; Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
  • Wilde C; Lallemand Inc., Montreal, QC, Canada.
  • Kuijpers NGA; Heineken Supply Chain, Zoeterwoude, Netherlands.
  • Geertman JM; Heineken Supply Chain, Zoeterwoude, Netherlands.
  • Wolfe KH; School of Medicine, UCD Conway Institute, University College Dublin, Dublin, Ireland.
  • Morrissey JP; School of Microbiology, Centre for Synthetic Biology and Biotechnology, Environmental Research Institute, APC Microbiome Institute, University College Cork, Cork, Ireland.
Front Genet ; 9: 94, 2018.
Article en En | MEDLINE | ID: mdl-29619042
Kluyveromyces marxianus is traditionally associated with fermented dairy products, but can also be isolated from diverse non-dairy environments. Because of thermotolerance, rapid growth and other traits, many different strains are being developed for food and industrial applications but there is, as yet, little understanding of the genetic diversity or population genetics of this species. K. marxianus shows a high level of phenotypic variation but the only phenotype that has been clearly linked to a genetic polymorphism is lactose utilisation, which is controlled by variation in the LAC12 gene. The genomes of several strains have been sequenced in recent years and, in this study, we sequenced a further nine strains from different origins. Analysis of the Single Nucleotide Polymorphisms (SNPs) in 14 strains was carried out to examine genome structure and genetic diversity. SNP diversity in K. marxianus is relatively high, with up to 3% DNA sequence divergence between alleles. It was found that the isolates include haploid, diploid, and triploid strains, as shown by both SNP analysis and flow cytometry. Diploids and triploids contain long genomic tracts showing loss of heterozygosity (LOH). All six isolates from dairy environments were diploid or triploid, whereas 6 out 7 isolates from non-dairy environment were haploid. This also correlated with the presence of functional LAC12 alleles only in dairy haplotypes. The diploids were hybrids between a non-dairy and a dairy haplotype, whereas triploids included three copies of a dairy haplotype.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Genet Año: 2018 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Genet Año: 2018 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Suiza