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Phenethyl Isothiocyanate Induces Apoptotic Cell Death Through the Mitochondria-dependent Pathway in Gefitinib-resistant NCI-H460 Human Lung Cancer Cells In Vitro.
Hsia, Te-Chun; Huang, Yi-Ping; Jiang, Yi-Wen; Chen, Hsin-Yu; Cheng, Zheng-Yu; Hsiao, Yung-Ting; Chen, Cheng-Yen; Peng, Shu-Fen; Chueh, Fu-Shin; Chou, Yu-Cheng; Chung, Jing-Gung.
Afiliación
  • Hsia TC; Department of Respiratory Therapy, China Medical University, Taichung, Taiwan, R.O.C.
  • Huang YP; Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.
  • Jiang YW; Department of Physiology, College of Medicine, China Medical University, Taichung, Taiwan, R.O.C.
  • Chen HY; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C.
  • Cheng ZY; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C.
  • Hsiao YT; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
  • Chen CY; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
  • Peng SF; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
  • Chueh FS; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C.
  • Chou YC; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
  • Chung JG; Division of Neurosurgical Oncology, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw chouycns@yahoo.com.tw.
Anticancer Res ; 38(4): 2137-2147, 2018 04.
Article en En | MEDLINE | ID: mdl-29599332
BACKGROUND/AIM: Some lung cancer patients treated with gefitinib develop resistance to this drug resulting in unsatisfactory treatment outcomes. Phenethyl isothiocyanate (PEITC), present in our common cruciferous vegetables, exhibits anticancer activities in many human cancer cell lines. Currently, there is no available information on the possible modification of gefitinib resistance of lung cancer in vitro by PEITC. Thus, the effects of PEITC on gefitinib resistant lung cancer NCI-H460 cells were investigated in vitro. MATERIALS AND METHODS: The total cell viability, apoptotic cell death, production of reactive oxygen species (ROS) and Ca2+, levels of mitochondria membrane potential (ΔΨm) and caspase-3, -8 and -9 activities were measured by flow cytometry assay. PEITC induced chromatin condensation was examined by DAPI staining. RESULTS: PEITC-induced cell morphological changes, decreased total viable cell number and induced apoptotic cell death in NCI-H460 and NCI-H460/G cells. PEITC decreased ROS production in NCI-H460 cells, but increased production in NCI-H460/G cells. PEITC increased Ca2+ production, decreased the levels of ΔΨm and increased caspase-3, -8 and -9 activities in both NCI-H460 and NCI-H460/G cells. Western blotting was used to examine the effect of apoptotic cell death associated protein expression in NCI-H460 NCI-H460/G cells after exposure to PEITC. Results showed that PEITC increased expression of cleaved caspase-3, PARP, GADD153, Endo G and pro-apoptotic protein Bax in NCI-H460/G cells. CONCLUSION: Based on these results, we suggest that PEITC induces apoptotic cell death via the caspase- and mitochondria-dependent pathway in NCI-H460/G cells.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quinazolinas / Apoptosis / Isotiocianatos / Resistencia a Antineoplásicos / Neoplasias Pulmonares / Mitocondrias Límite: Humans Idioma: En Revista: Anticancer Res Año: 2018 Tipo del documento: Article Pais de publicación: Grecia
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quinazolinas / Apoptosis / Isotiocianatos / Resistencia a Antineoplásicos / Neoplasias Pulmonares / Mitocondrias Límite: Humans Idioma: En Revista: Anticancer Res Año: 2018 Tipo del documento: Article Pais de publicación: Grecia