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The detection and prevalence of leukotoxin gene variant strains of Fusobacterium necrophorum in footrot lesions of sheep in Kashmir, India.
Farooq, S; Wani, S A; Hassan, M N; Aalamgeer, S; Kashoo, Z A; Magray, S N; Bhat, M A.
Afiliación
  • Farooq S; KVK-Kupwara, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Jammu and Kashmir, India. Electronic address: shaheen_mvsc@yahoo.co.in.
  • Wani SA; Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India.
  • Hassan MN; KVK-Nyoma, SKUAST-K, Jammu and Kashmir, India.
  • Aalamgeer S; Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India.
  • Kashoo ZA; Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India.
  • Magray SN; Division of Veterinary Microbiology and Immunology, SKUAST-K, Shuhama (Alusteng), Srinagar, 190006, Jammu and Kashmir, India.
  • Bhat MA; Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), R.S.Pura, Jammu and Kashmir, India.
Anaerobe ; 51: 36-41, 2018 Jun.
Article en En | MEDLINE | ID: mdl-29596988
The objective of this study was to determine the prevalence and identification of leukotoxin gene, lktA, variant strains of Fusobacterium necrophorum in the footrot lesions of sheep. The detection of F. necrophorum was carried out by PCR targeting the lktA gene fragment and identification of lktA variant strains was done by PCR-single stranded conformational polymorphism (PCR-SSCP) and gene sequencing. Of the 450 swabs collected from footrot lesions of sheep, 117 were lktA-positive for F. necrophorum. Of the 50 swabs collected from apparently asymptomatic sheep, only one was lktA-positive for F. necrophorum. The overall prevalence of F. necrophorum in footrot affected sheep in Kashmir valley was 26%, and ranged from 20 to 34.8%, respectively. PCR-SSCP of lktA gene fragment analysis revealed three lktA variants, designated as JKS-F1/F2/F3, while two samples (1.7%) showed multiple lktA variant strains of F. necrophorum in a single footrot-affected sheep hoof. This appears to be the first report on the presence of more than one lktA variant of F. necrophorum in a footrot lesion of sheep. The JKS-F3 lktA variant was the most frequent (75.4%), followed by JKS-F2 (14.4%) and JKS-F1 (8.4%), respectively. Among the three lktA variants identified, JKS-F3 was detected in 74 (86.0%) samples from severe footrot affected sheep with a lesion score of 4. The data suggest that JKS-F3 is the predominant lktA variant of F. necrophorum and is associated with severe footrot in sheep. Hence, JKS-F3 may be a significant variant contributing to the severity and duration of the disease in sheep.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Ovejas / Portador Sano / Polimorfismo Conformacional Retorcido-Simple / Exotoxinas / Infecciones por Fusobacterium / Fusobacterium necrophorum Tipo de estudio: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Anaerobe Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Ovejas / Portador Sano / Polimorfismo Conformacional Retorcido-Simple / Exotoxinas / Infecciones por Fusobacterium / Fusobacterium necrophorum Tipo de estudio: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Anaerobe Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido