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Zebrafish: A Pharmacogenetic Model for Anesthesia.
Bedell, Victoria; Buglo, Elena; Marcato, Daniel; Pylatiuk, Christian; Mikut, Ralf; Stegmaier, Johannes; Scudder, Will; Wray, Maxwell; Züchner, Stephan; Strähle, Uwe; Peravali, Ravindra; Dallman, Julia E.
Afiliación
  • Bedell V; Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, United States. Electronic address: Victoria.bedell@uphs.upenn.edu.
  • Buglo E; John P. Hussman Institute for Human Genomics, University of Miami, Miami, FL, United States; Dr. John T. MacDonald Foundation, University of Miami, Miami, FL, United States; University of Miami, Coral Gables, FL, United States.
  • Marcato D; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Pylatiuk C; Institute of Applied Computer Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Mikut R; Institute of Applied Computer Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Stegmaier J; Institute of Applied Computer Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Scudder W; University of Miami, Coral Gables, FL, United States.
  • Wray M; University of Miami, Coral Gables, FL, United States.
  • Züchner S; John P. Hussman Institute for Human Genomics, University of Miami, Miami, FL, United States; Dr. John T. MacDonald Foundation, University of Miami, Miami, FL, United States.
  • Strähle U; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Peravali R; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany.
  • Dallman JE; University of Miami, Coral Gables, FL, United States.
Methods Enzymol ; 602: 189-209, 2018.
Article en En | MEDLINE | ID: mdl-29588029
General anesthetics are small molecules that interact with and effect the function of many different proteins to promote loss of consciousness, amnesia, and sometimes, analgesia. Owing to the complexity of this state transition and the transient nature of these drug/protein interactions, anesthetics can be difficult to study. The zebrafish is an emerging model for the discovery of both new genes required for the response to and side effects of anesthesia. Here we discuss the tools available to manipulate the zebrafish genome, including both genetic screens and genome engineering approaches. Additionally, there are various robust behavior assays available to study anesthetic and other drug responses. These assays are available for single-gene study or high throughput for genetic or drug discovery. Finally, we present a case study of using propofol as an anesthetic in the zebrafish. These techniques and protocols make the zebrafish a powerful model to study anesthetic mechanisms and drug discovery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacogenética / Pez Cebra / Ensayos Analíticos de Alto Rendimiento / Anestesia / Anestésicos Límite: Animals / Humans Idioma: En Revista: Methods Enzymol Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacogenética / Pez Cebra / Ensayos Analíticos de Alto Rendimiento / Anestesia / Anestésicos Límite: Animals / Humans Idioma: En Revista: Methods Enzymol Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos