Mitochondrial fragmentation affects neither the sensitivity to TNF&#945;-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria.

Lobet, Elodie; Willemart, Kevin; Ninane, Noëlle; Demazy, Catherine; Sedzicki, Jaroslaw; Lelubre, Christophe; De Bolle, Xavier; Renard, Patricia; Raes, Martine; Dehio, Christoph; Letesson, Jean-Jacques; Arnould, Thierry

Mitochondrial fragmentation affects neither the sensitivity to TNFα-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria.

Lobet, Elodie; Willemart, Kevin; Ninane, Noëlle; Demazy, Catherine; Sedzicki, Jaroslaw; Lelubre, Christophe; De Bolle, Xavier; Renard, Patricia; Raes, Martine; Dehio, Christoph; Letesson, Jean-Jacques; Arnould, Thierry.

Afiliación

Lobet E; Laboratory of Biochemistry and Cell Biology (URBC, Unité de Recherche en Biologie Cellulaire)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Willemart K; Microorganisms Biology Research Unit (URBM, Unité de Recherche en Biologie des Microorganismes)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Ninane N; Laboratory of Biochemistry and Cell Biology (URBC, Unité de Recherche en Biologie Cellulaire)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Demazy C; Laboratory of Biochemistry and Cell Biology (URBC, Unité de Recherche en Biologie Cellulaire)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Sedzicki J; Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056, Basel, Switzerland.
Lelubre C; Laboratory of Experimental Medicine (ULB 222 Unit), Medicine Faculty, Université Libre de Bruxelles, CHU de Charleroi, Rue de Gozée 706, 6110, Montigny-le-Tilleul, Belgium.
De Bolle X; Microorganisms Biology Research Unit (URBM, Unité de Recherche en Biologie des Microorganismes)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Renard P; Laboratory of Biochemistry and Cell Biology (URBC, Unité de Recherche en Biologie Cellulaire)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Raes M; Laboratory of Biochemistry and Cell Biology (URBC, Unité de Recherche en Biologie Cellulaire)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Dehio C; Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056, Basel, Switzerland.
Letesson JJ; Microorganisms Biology Research Unit (URBM, Unité de Recherche en Biologie des Microorganismes)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Arnould T; Laboratory of Biochemistry and Cell Biology (URBC, Unité de Recherche en Biologie Cellulaire)-NARILIS (Namur Research Institute for Life Sciences), University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium. thierry.arnould@unamur.be.

Sci Rep ; 8(1): 5173, 2018 03 26.

Article en En | MEDLINE | ID: mdl-29581535

RESUMEN

Mitochondria are complex organelles that participate in many cellular functions, ranging from ATP production to immune responses against viruses and bacteria. This integration of a plethora of functions within a single organelle makes mitochondria a very attractive target to manipulate for intracellular pathogens. We characterised the crosstalk that exists between Brucella abortus, the causative agent of brucellosis, and the mitochondria of infected cells. Brucella replicates in a compartment derived from the endoplasmic reticulum (ER) and modulates ER functionality by activating the unfolded protein response. However, the impact of Brucella on the mitochondrial population of infected cells still requires a systematic study. We observed physical contacts between Brucella containing vacuoles and mitochondria. We also found that B. abortus replication is independent of mitochondrial oxidative phosphorylation and that mitochondrial reactive oxygen species do not participate to the control of B. abortus infection in vitro. We demonstrated that B. abortus and B. melitensis induce a drastic mitochondrial fragmentation at 48 hours post-infection in different cell types, including myeloid and non-myeloid cells. This fragmentation is DRP1-independent and might be caused by a deficit of mitochondrial fusion. However, mitochondrial fragmentation does not change neither Brucella replication efficiency, nor the susceptibility of infected cells to TNFα-induced apoptosis.

Asunto(s)

Brucella abortus/genética; Brucelosis/genética; Dinaminas/genética; Factor de Necrosis Tumoral alfa/genética; Animales; Apoptosis/genética; Brucella abortus/patogenicidad; Brucelosis/microbiología; Brucelosis/patología; Retículo Endoplásmico/genética; Retículo Endoplásmico/microbiología; Humanos; Macrófagos/metabolismo; Macrófagos/patología; Ratones; Mitocondrias/genética; Mitocondrias/microbiología; Células RAW 264.7; Especies Reactivas de Oxígeno/metabolismo; Respuesta de Proteína Desplegada/genética; Vacuolas/genética

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brucella abortus / Brucelosis / Factor de Necrosis Tumoral alfa / Dinaminas Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido

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