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Electrochemical Measurement of Dopamine Release and Uptake in Zebrafish Following Treatment with Carboplatin.
Field, Thomas M; Shin, Mimi; Stucky, Chase S; Loomis, Joseph; Johnson, Michael A.
Afiliación
  • Field TM; Department of Chemistry and Graduate Program in Neuroscience, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA.
  • Shin M; Department of Chemistry and Graduate Program in Neuroscience, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA.
  • Stucky CS; Department of Chemistry and Graduate Program in Neuroscience, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA.
  • Loomis J; Department of Chemistry, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA.
  • Johnson MA; Department of Chemistry and Graduate Program in Neuroscience, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA.
Chemphyschem ; 19(10): 1192-1196, 2018 05 22.
Article en En | MEDLINE | ID: mdl-29573086
Post-chemotherapy cognitive impairment, also known as 'chemobrain,' is a neurological condition in which cognitive function is impaired as a result of cancer chemotherapy treatment. In this work, we used fast-scan cyclic voltammetry (FSCV) to measure electrically evoked dopamine release and uptake in whole brain preparations from zebrafish that have been treated with carboplatin, an agent associated with chemobrain. We administered carboplatin by addition to the fish's tank water or their food. One week of treatment with 100 µM carboplatin in the water was needed to significantly impair dopamine release (∼40 % of control); however, only one day of treatment through the zebrafish's food was needed to cause a similar impairment. Atomic absorption spectroscopy measurements suggested that administration through food resulted in higher initial levels of carboplatin compared to water administration, but water administration resulted in an increase over time. Uptake, determined by modeling stimulated release plots, was unaffected. These results are consistent with our previous findings of diminished neurotransmitter release in rats and support a role for zebrafish in chemobrain-related studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Carboplatino / Técnicas Electroquímicas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Chemphyschem Asunto de la revista: BIOFISICA / QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Carboplatino / Técnicas Electroquímicas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Chemphyschem Asunto de la revista: BIOFISICA / QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania