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Transcription factor FoxM1 is the downstream target of c-Myc and contributes to the development of prostate cancer.
Pan, Huafeng; Zhu, Yudi; Wei, Wei; Shao, Siliang; Rui, Xin.
Afiliación
  • Pan H; Department of Urology, Ningbo No.2 Hospital, No.41 Xibei Street, Ningbo, 315010, Zhejiang Province, People's Republic of China.
  • Zhu Y; Department of Urology, Ningbo No.2 Hospital, No.41 Xibei Street, Ningbo, 315010, Zhejiang Province, People's Republic of China.
  • Wei W; Department of Urology, Ningbo No.2 Hospital, No.41 Xibei Street, Ningbo, 315010, Zhejiang Province, People's Republic of China.
  • Shao S; Department of Urology, Ningbo No.2 Hospital, No.41 Xibei Street, Ningbo, 315010, Zhejiang Province, People's Republic of China.
  • Rui X; Department of Urology, Ningbo No.2 Hospital, No.41 Xibei Street, Ningbo, 315010, Zhejiang Province, People's Republic of China. xinrui_nb@163.com.
World J Surg Oncol ; 16(1): 59, 2018 Mar 20.
Article en En | MEDLINE | ID: mdl-29554906
BACKGROUND: Prostate cancer is a common malignancy and the second leading cause of cancer death in men. Elevated expression of the transcription factor FoxM1 and c-Myc has been identified in prostate cancer. However, the potential mechanism of elevated FoxM1 and c-Myc to the development of prostate cancer has not been identified. METHODS: In this report, the mRNA level of FoxM1 and c-Myc was detected in 30 prostate cancer and para-cancer tissues. Then, we detected the expression level of FoxM1 by real-time PCR and Western blot after disturbance of the expression level of c-Myc in PC-3 cells. Whether c-Myc could bind to FoxM1 promoter was identified by ChIP assay. Finally, the migratory, invasive, and proliferative abilities in FoxM1 overexpressing and silencing PC-3 cells were detected by wound healing, transwell assay, CCK-8 assays, and Ki-67 protein level. RESULTS: We found that the expression level of FoxM1 and c-Myc were both increased in prostate cancer samples compared with para-cancer samples. The expression level of FoxM1 was changed consistent with the protein level of c-Myc. ChIP assay detected the direct binding of c-Myc in FoxM1 gene promoter. Lastly, overexpression of FoxM1 increased the migratory, invasive, and proliferative abilities of PC-3 cells, and its downregulation significantly decreased the migratory, invasive, and proliferative abilities. CONCLUSIONS: In conclusion, FoxM1 was significantly increased in prostate cancer samples, and it could regulate the proliferative and invasive ability of prostate cancer cells which might be a new target for prostate cancer. Besides, c-Myc could regulate the expression level of FoxM1 by directly binding to its gene promoter.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / Movimiento Celular / Proteínas Proto-Oncogénicas c-myc / Proteína Forkhead Box M1 Tipo de estudio: Prognostic_studies Límite: Humans / Male Idioma: En Revista: World J Surg Oncol Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Biomarcadores de Tumor / Regulación Neoplásica de la Expresión Génica / Movimiento Celular / Proteínas Proto-Oncogénicas c-myc / Proteína Forkhead Box M1 Tipo de estudio: Prognostic_studies Límite: Humans / Male Idioma: En Revista: World J Surg Oncol Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido