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Protein Engineered Triblock Polymers Composed of Two SADs: Enhanced Mechanical Properties and Binding Abilities.
Olsen, Andrew J; Katyal, Priya; Haghpanah, Jennifer S; Kubilius, Matthew B; Li, Ruipeng; Schnabel, Nicole L; O'Neill, Sean C; Wang, Yao; Dai, Min; Singh, Navjot; Tu, Raymond S; Montclare, Jin Kim.
Afiliación
  • Olsen AJ; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • Katyal P; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • Haghpanah JS; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • Kubilius MB; Chemical Engineering Department , City College of New York , 160 Convent Avenue , New York , New York 10031 , United States.
  • Li R; National Synchrotron Light Source II , Brookhaven National Laboratory , Upton , New York 11973 , United States.
  • Schnabel NL; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • O'Neill SC; Chemical Engineering Department , City College of New York , 160 Convent Avenue , New York , New York 10031 , United States.
  • Wang Y; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • Dai M; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • Singh N; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
  • Tu RS; Chemical Engineering Department , City College of New York , 160 Convent Avenue , New York , New York 10031 , United States.
  • Montclare JK; Chemical and Biomolecular Engineering Department , New York University Tandon School of Engineering , 6 Metrotech Center , Brooklyn , New York 11201 , United States.
Biomacromolecules ; 19(5): 1552-1561, 2018 05 14.
Article en En | MEDLINE | ID: mdl-29544048
Recombinant methods have been used to engineer artificial protein triblock polymers composed of two different self-assembling domains (SADs) bearing one elastin (E) flanked by two cartilage oligomeric matrix protein coiled-coil (C) domains to generate CEC. To understand how the two C domains improve small molecule recognition and the mechanical integrity of CEC, we have constructed CL44AECL44A, which bears an impaired CL44A domain that is unstructured as a negative control. The CEC triblock polymer demonstrates increased small molecule binding and ideal elastic behavior for hydrogel formation. The negative control CL44AECL44A does not exhibit binding to small molecule and is inelastic at lower temperatures, affirming the favorable role of C domain and its helical conformation. While both CEC and CL44AECL44A assemble into micelles, CEC is more densely packed with C domains on the surface enabling the development of networks leading to hydrogel formation. Such protein engineered triblock copolymers capable of forming robust hydrogels hold tremendous promise for biomedical applications in drug delivery and tissue engineering.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elastina / Elasticidad / Proteína de la Matriz Oligomérica del Cartílago Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elastina / Elasticidad / Proteína de la Matriz Oligomérica del Cartílago Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos