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Identification and validation of reference genes for qRT-PCR analysis in mulberry (Morus alba L.).
Dai, Fanwei; Zhao, Xiting; Tang, Cuiming; Wang, Zhenjiang; Kuang, Zheshi; Li, Zhiyi; Huang, Jing; Luo, Guoqing.
Afiliación
  • Dai F; Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Zhao X; Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture, Guangzhou, China.
  • Tang C; College of Life Sciences, Henan Normal University, Xinxiang, China.
  • Wang Z; Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Kuang Z; Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture, Guangzhou, China.
  • Li Z; Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Huang J; Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture, Guangzhou, China.
  • Luo G; Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
PLoS One ; 13(3): e0194129, 2018.
Article en En | MEDLINE | ID: mdl-29543877
Mulberry (Morus alba L.) is an important economic tree species in many countries. Quantitative real time PCR (qRT-PCR) has become a widely used method for gene expression studies in plants. A suitable reference gene is essential to ensure accurate and reliable results for qRT-PCR analyses. However, no reports describing the selection of reference genes have been published for mulberry. In this work, we evaluated the stability of twenty candidate reference genes in different plant tissues and under different stress conditions by qRT-PCR in mulberry using algorithms in two programs-geNorm and NormFinder. The results revealed that TUB2, UBI4, ACTIN3 and RPL4 were ranked as the most stable reference genes in the samples subsets, whereas EF1α4 and TUB3showed the least stability with both algorithms. To further validate the stability of the reference genes, the expression patterns of six genes of mulberry were analyzed by normalization with the selected reference genes. Our study will benefit future analyses of gene expression in mulberry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes de Plantas / Morus Tipo de estudio: Diagnostic_studies Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes de Plantas / Morus Tipo de estudio: Diagnostic_studies Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos