TGF-ß1 promotes cell barrier function upon maturation of corneal endothelial cells.
Sci Rep
; 8(1): 4438, 2018 03 13.
Article
en En
| MEDLINE
| ID: mdl-29535350
Human corneal endothelial cells (HCECs) easily become fibroblastic-like when cultured, rendering them unsuitable for tissue engineering of the cornea. Transforming growth factor ß (TGF-ß) could be a key factor in this phenomenon; however, TGF-ß is also known to maintain the endothelium in a quiescent state in vivo. This work aimed to compare the effects of TGF-ß1 on the phenotype of HCECs during the proliferation and maturation phases. Our results show that addition of TGF-ß1 during the active proliferation phase produced fibroblastic HCECs and loss of the cell junction markers ZO-1 and n-cadherin, independent from the presence of epidermal growth factor (EGF). By contrast, addition of TGF-ß1 in maturation media containing few mitogens led to an endothelial phenotype and functional cell junctions as HCECs developed a high trans-endothelial resistance. Furthermore, addition of AG-1478, an epithelial growth factor receptor inhibitor, enhanced the gain of the endothelial phenotype and cell barrier function. Overall, these results show that TGF-ß1 can be used to promote the formation of a typical leaky endothelial barrier during the maturation phase of cultured HCECs. A two-phase culture of HCECs using distinct proliferation and maturation media could also be key for developing ideal HCEC culture conditions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Benzamidas
/
Endotelio Corneal
/
Técnicas de Cultivo de Célula
/
Dioxoles
/
Factor de Crecimiento Transformador beta1
Límite:
Adult
/
Aged
/
Humans
/
Middle aged
Idioma:
En
Revista:
Sci Rep
Año:
2018
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Reino Unido