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TGF-ß1 promotes cell barrier function upon maturation of corneal endothelial cells.
Beaulieu Leclerc, Véronique; Roy, Olivier; Santerre, Kim; Proulx, Stéphanie.
Afiliación
  • Beaulieu Leclerc V; Centre de recherche du Centre hospitalier universitaire (CHU) de Québec - Université Laval, axe médecine régénératrice, Hôpital du Saint-Sacrement, Québec, QC, Canada.
  • Roy O; Centre d'organogénèse expérimentale de l'Université Laval/LOEX, Québec, QC, Canada.
  • Santerre K; Département d'Ophtalmologie, Faculté de médecine, Université Laval, Québec, QC, Canada.
  • Proulx S; Centre de recherche du Centre hospitalier universitaire (CHU) de Québec - Université Laval, axe médecine régénératrice, Hôpital du Saint-Sacrement, Québec, QC, Canada.
Sci Rep ; 8(1): 4438, 2018 03 13.
Article en En | MEDLINE | ID: mdl-29535350
Human corneal endothelial cells (HCECs) easily become fibroblastic-like when cultured, rendering them unsuitable for tissue engineering of the cornea. Transforming growth factor ß (TGF-ß) could be a key factor in this phenomenon; however, TGF-ß is also known to maintain the endothelium in a quiescent state in vivo. This work aimed to compare the effects of TGF-ß1 on the phenotype of HCECs during the proliferation and maturation phases. Our results show that addition of TGF-ß1 during the active proliferation phase produced fibroblastic HCECs and loss of the cell junction markers ZO-1 and n-cadherin, independent from the presence of epidermal growth factor (EGF). By contrast, addition of TGF-ß1 in maturation media containing few mitogens led to an endothelial phenotype and functional cell junctions as HCECs developed a high trans-endothelial resistance. Furthermore, addition of AG-1478, an epithelial growth factor receptor inhibitor, enhanced the gain of the endothelial phenotype and cell barrier function. Overall, these results show that TGF-ß1 can be used to promote the formation of a typical leaky endothelial barrier during the maturation phase of cultured HCECs. A two-phase culture of HCECs using distinct proliferation and maturation media could also be key for developing ideal HCEC culture conditions.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Benzamidas / Endotelio Corneal / Técnicas de Cultivo de Célula / Dioxoles / Factor de Crecimiento Transformador beta1 Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Benzamidas / Endotelio Corneal / Técnicas de Cultivo de Célula / Dioxoles / Factor de Crecimiento Transformador beta1 Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido