Your browser doesn't support javascript.
loading
Immunophenotypic analysis of the chronological events of tissue repair in aortic medial dissections.
Visonà, Silvia D; de Boer, Onno J; Mackaaij, Claire; de Boer, Hans H; Pertiwi, Kartika R; de Winter, Ruben W; Osculati, Antonio; van der Wal, Allard C.
Afiliación
  • Visonà SD; Dept. of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy.
  • de Boer OJ; Dept. of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands.
  • Mackaaij C; Dept. of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands.
  • de Boer HH; Dept. of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands.
  • Pertiwi KR; Dept. of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands; Dept. of Biology Education, Faculty of Mathematics and Natural Science, Yogyakarta State University, Indonesia.
  • de Winter RW; Dept. of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands.
  • Osculati A; Dept. of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 12, 27100 Pavia, Italy.
  • van der Wal AC; Dept. of Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105, AZ, Amsterdam, the Netherlands. Electronic address: a.c.vanderwal@amc.uva.nl.
Cardiovasc Pathol ; 34: 9-14, 2018.
Article en En | MEDLINE | ID: mdl-29525729
Acute medial dissection of aorta can occur in the context of a sudden and unexpected death. For medico-legal reasons it is important to estimate as accurately the histological age of dissections. We evaluated the additional value of a systematic application of immunohistochemistry, compared with conventional histology only, in determining chronological steps of injury and repair. Thirty two paraffin embedded specimens of aortic dissection were retrospectively allocated to one of four defined stages: acute (I), subacute (II), early organizing (III) and scarring (IV) using Hematoxylin and Eosin and Elastica van Gieson stained sections. Subsequent immunohistochemically staining was performed with the following markers: (myeloperoxidase (neutrophils), citrullinated-Histone 3 (neutrophil extracellular traps), CD68 (macrophages), CD3 (T-cells), CD31 and CD34 (endothelial cells), and smooth muscle actin. Immune stained sections were scored semi-quantitatively. Histologically, five cases were identified as stage I, 16 as II, 7 as III and 4 as IV. Additional immunostaining for smooth muscle cells and endothelial cells altered the classification in 25% of cases (all in groups II and III). Immunostaining and semi-quantitative grading of involvement of neutrophils, macrophages and NETs also provided specific distribution patterns over the 4 age categories, including unexpected involvement of the peri adventitial fat tissue. In conclusion, it appears that semi-quantitative immunohistochemistry of resident vascular wall cells, inflammatory cells and NETS represents a useful adjunct in detailed histopathological grading of the chronological age of aortic dissections.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aorta / Aneurisma de la Aorta / Inmunofenotipificación / Túnica Media / Remodelación Vascular / Disección Aórtica Tipo de estudio: Observational_studies / Prognostic_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Cardiovasc Pathol Asunto de la revista: ANGIOLOGIA / CARDIOLOGIA / PATOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aorta / Aneurisma de la Aorta / Inmunofenotipificación / Túnica Media / Remodelación Vascular / Disección Aórtica Tipo de estudio: Observational_studies / Prognostic_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Cardiovasc Pathol Asunto de la revista: ANGIOLOGIA / CARDIOLOGIA / PATOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos