Your browser doesn't support javascript.
loading
Microfluidic liquid-air dual-gradient chip for synergic effect bio-evaluation of air pollutant.
Liu, Xian-Jun; Hu, Shan-Wen; Xu, Bi-Yi; Zhao, Ge; Li, Xiang; Xie, Fu-Wei; Xu, Jing-Juan; Chen, Hong-Yuan.
Afiliación
  • Liu XJ; Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, People's Republic of China.
  • Hu SW; State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Centre of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, People's Republic of China.
  • Xu BY; State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Centre of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, People's Republic of China. Electronic address: xuby@nju.edu.cn.
  • Zhao G; Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, People's Republic of China.
  • Li X; Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, People's Republic of China.
  • Xie FW; Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, People's Republic of China. Electronic address: xiefuwei@sina.com.
  • Xu JJ; State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Centre of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, People's Republic of China.
  • Chen HY; State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Centre of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, People's Republic of China.
Talanta ; 182: 202-209, 2018 May 15.
Article en En | MEDLINE | ID: mdl-29501141
In this paper, a novel prototype liquid-air dual gradient chip is introduced, which has paved the way for effective synergic effect bio-evaluation of air pollutant. The chip is composed of an array of the agarose liquid-air interfaces, top air gradient layer and bottom liquid gradient layer. The novel agarose liquid-air interface allows for non-biased exposure of cells to all the substances in the air and diffusive interactions with the liquid phase; while the dual liquid-air gradient provides powerful screening abilities, which well reduced errors, saved time and cost from repeated experiment. Coupling the two functions, the chip subsequently facilitates synergic effect evaluation of both liquid and air factors on cells. Here cigarette smoke was taken as the model air pollutant, and its strong synergic effects with inflammatory level of A549 lung cancer cells on their fate were successfully quantified for the first time. These results well testified that the proposed dual-gradient chip is powerful and indispensable for bio-evaluation of air pollutant.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Humo / Contaminación por Humo de Tabaco / Contaminantes Atmosféricos / Dispositivos Laboratorio en un Chip Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Talanta Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Humo / Contaminación por Humo de Tabaco / Contaminantes Atmosféricos / Dispositivos Laboratorio en un Chip Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Talanta Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos