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LINE-1 ORF1 Protein Is Up-regulated by Reactive Oxygen Species and Associated with Bladder Urothelial Carcinoma Progression.
Whongsiri, Patcharawalai; Pimratana, Chaowat; Wijitsettakul, Udomsak; Jindatip, Depicha; Sanpavat, Anapat; Schulz, Wolfgang A; Hoffmann, Michèle J; Goering, Wolfgang; Boonla, Chanchai.
Afiliación
  • Whongsiri P; Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Pimratana C; Division of Urology, Buriram Hospital, Buriram, Thailand.
  • Wijitsettakul U; Division of Urology, Buriram Hospital, Buriram, Thailand.
  • Jindatip D; Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Sanpavat A; Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Schulz WA; Department of Urology, Medical Faculty, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
  • Hoffmann MJ; Department of Urology, Medical Faculty, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
  • Goering W; Department of Pathology, Medical Faculty, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
  • Boonla C; Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand chanchai.b@chula.ac.th.
Cancer Genomics Proteomics ; 15(2): 143-151, 2018.
Article en En | MEDLINE | ID: mdl-29496693
BACKGROUND/AIM: Reactivation of long interspersed nuclear element-1 (LINE-1) and oxidative stress are suggested to have oncogenic potential to drive tumorigenesis and cancer progression. We previously demonstrated that reactive oxygen species (ROS) caused hypomethylation of LINE-1 elements in bladder cancer cells. In this study, we investigated the expression of LINE-1-encoded protein (ORF1p) and oxidative stress marker 4-hydroxynonenal (4-HNE) in human bladder cancer tissues, as well as induction of ORF1p expression by ROS in bladder cancer cell lines. MATERIALS AND METHODS: Thirty-six cancerous and 15 non-cancerous adjacent tissues were immunohistochemically stained for ORF1p and 4-HNE. ORF1p expression and cell migration were determined in bladder cancer cells exposed to H2O2 Results: ORF1p and 4-HNE expression was higher in cancerous than non-cancerous tissues. Elevated ORF1p expression was associated with increased 4-HNE expression and with advanced tumors. H2O2 provoked oxidative stress and up-regulated ORF1p expression in VM-CUB-1 compared to the untreated control, and to a lesser degree in TCCSUP. H2O2 exposure enhanced cell migration in UM-UC-3, TCCSUP and VM-CUB-1. CONCLUSION: Elevated ORF1p expression is associated with tumor progression. ROS experimentally induce ORF1p expression and promote migration in bladder cancer cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Proteínas / Especies Reactivas de Oxígeno / Elementos de Nucleótido Esparcido Largo Tipo de estudio: Risk_factors_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Cancer Genomics Proteomics Asunto de la revista: BIOQUIMICA / GENETICA MEDICA / NEOPLASIAS Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Grecia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Proteínas / Especies Reactivas de Oxígeno / Elementos de Nucleótido Esparcido Largo Tipo de estudio: Risk_factors_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Cancer Genomics Proteomics Asunto de la revista: BIOQUIMICA / GENETICA MEDICA / NEOPLASIAS Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Grecia