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Assessment of DNA damages in lymphocytes of agricultural workers exposed to pesticides by comet assay in a cross-sectional study.
Intranuovo, Graziana; Schiavulli, Nunzia; Cavone, Domenica; Birtolo, Francesco; Cocco, Pierluigi; Vimercati, Luigi; Macinagrossa, Linda; Giordano, Annamaria; Perrone, Tommasina; Ingravallo, Giuseppe; Mazza, Patrizio; Strusi, Michela; Spinosa, Caterina; Specchia, Giorgina; Ferri, Giovanni M.
Afiliación
  • Intranuovo G; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
  • Schiavulli N; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
  • Cavone D; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
  • Birtolo F; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
  • Cocco P; b Department of Public Health , Clinic and Molecular Medicine, Section of Occupational Medicine, Regional University Hospital, School of Medicine, University of Cagliari , Monserrato (Cagliari) , Italy.
  • Vimercati L; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
  • Macinagrossa L; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
  • Giordano A; c Department of Haematology and Organ Transplantation (DETO) , University Hospital "Policlinico", Unit of Haematology, School of Medicine, University of Bari , Bari , Italy.
  • Perrone T; c Department of Haematology and Organ Transplantation (DETO) , University Hospital "Policlinico", Unit of Haematology, School of Medicine, University of Bari , Bari , Italy.
  • Ingravallo G; c Department of Haematology and Organ Transplantation (DETO) , University Hospital "Policlinico", Unit of Haematology, School of Medicine, University of Bari , Bari , Italy.
  • Mazza P; d ASL of Taranto, Moscati Hospital, Unit of Haematology , Taranto , Italy.
  • Strusi M; d ASL of Taranto, Moscati Hospital, Unit of Haematology , Taranto , Italy.
  • Spinosa C; d ASL of Taranto, Moscati Hospital, Unit of Haematology , Taranto , Italy.
  • Specchia G; c Department of Haematology and Organ Transplantation (DETO) , University Hospital "Policlinico", Unit of Haematology, School of Medicine, University of Bari , Bari , Italy.
  • Ferri GM; a Interdisciplinary Department of Medicine (DIM), Section "B. Ramazzini" , University Hospital "Policlinico", Unit of Occupational Medicine, School of Medicine, University of Bari , Bari , Italy.
Biomarkers ; 23(5): 462-473, 2018 Jul.
Article en En | MEDLINE | ID: mdl-29493297
PURPOSE: To assess the predictive power of the comet assay in the context of occupational exposure to pesticides. MATERIALS AND METHODS: The recruited subjects completed a structured questionnaire and gave a blood sample. Exposure to pesticides was measured by means of an algorithm based on Dosemeci's work (Agricultural Health Study). Approximately 50 images were analyzed for each sample via fluorescence microscopy. The extent of DNA damage was estimated by tail moment (TM) and is the product of tail DNA (%) and tail Length. RESULTS: Crude significant risks (odds ratios, ORs) for values higher than the 75th percentile of TM were observed among the exposed subjects (score > 1). The frequency of some confounding factors (sex, age and smoking) was significantly higher among the exposed workers. A significant dose-effect relationship was observed between TM and exposure score. Significant high-risk estimates (ORs), adjusted by the studied confounding factors, among exposure to pesticides and TM, % tail DNA and tail length were confirmed using unconditional logistic regression models. CONCLUSIONS: The adjusted associations (ORs) between the comet parameters and exposure to pesticides were significant. The sensitivity of the comet test was low (41%), the specificity (89%) and the predictive positive value (0.77) were found acceptable.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Daño del ADN / Linfocitos / Exposición Profesional / Ensayo Cometa / Agricultura Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Qualitative_research / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Biomarkers Asunto de la revista: BIOQUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Daño del ADN / Linfocitos / Exposición Profesional / Ensayo Cometa / Agricultura Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Qualitative_research / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Biomarkers Asunto de la revista: BIOQUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido