Your browser doesn't support javascript.
loading
In vivo screening platform for shiga toxin-producing Escherichia coli (STEC) using Caenorhabditis elegans as a model.
Hwang, Su-Bin; Choi, Jung-Gu; Wei, Shuai; Park, Byung-Jae; Chelliah, Ramachandran; Oh, Deog-Hwan.
Afiliación
  • Hwang SB; Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Korea.
  • Choi JG; Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Korea.
  • Wei S; Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Korea.
  • Park BJ; Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Korea.
  • Chelliah R; Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Korea.
  • Oh DH; Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Korea.
PLoS One ; 13(2): e0193277, 2018.
Article en En | MEDLINE | ID: mdl-29489863
Shiga toxin-producing Escherichia coli (STEC) strains are the main cause of bacillary dysentery, although STEC strains generally induce milder disease symptoms compared to Shigella species. This study aimed to determine the virulence of STEC using the nematode Caenorhabditis elegans as a model host. Worm killing, fertility and bacterial colonisation assays were performed to examine the potential difference in the virulence of STEC strains compared to that of the control E. coli OP50 strains on which worms were fed. A statistically significant difference in the survival rates of C. elegans was observed in that the STEC strains caused death in 8-10 days and the E. coli OP50 strains caused death in 15 days. STEC strains severely reduced the fertility of the worms. The intestinal load of bacteria in the adult stage nematodes harbouring the E. coli OP50 strains was found to be 3.5 log CFU mL-1. In contrast, the STEC strains E15, E18 and E22 harboured 4.1, 4.2 and 4.7 log CFU ml-1 per nematode, respectively. The heat-killed STEC strains significantly increased the longevity of the worms compared to the non-heated STEC strains. In addition, PCR-based genomic profiling of shiga toxin genes, viz., stx1 and stx2, identified in selected STEC strains revealed that these toxins may be associated with the virulence of the STEC strains. This study demonstrated that C. elegans is an effective model to examine and compare the pathogenicity and virulence variation of STEC strains to that of E. coli OP50 strains.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caenorhabditis elegans / Modelos Animales de Enfermedad / Escherichia coli Shiga-Toxigénica / Longevidad Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caenorhabditis elegans / Modelos Animales de Enfermedad / Escherichia coli Shiga-Toxigénica / Longevidad Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article Pais de publicación: Estados Unidos