Your browser doesn't support javascript.
loading
Effects of long-term cadmium exposure on urinary metabolite profiles in mice.
Sarma, Sailendra Nath; Saleem, Ammar; Lee, Jin-Yong; Tokumoto, Maki; Hwang, Gi-Wook; Man Chan, Hing; Satoh, Masahiko.
Afiliación
  • Sarma SN; Department of Biology, University of Ottawa, Canada.
  • Saleem A; Department of Biology, University of Ottawa, Canada.
  • Lee JY; Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University.
  • Tokumoto M; Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University.
  • Hwang GW; Laboratory of Molecular Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University.
  • Man Chan H; Department of Biology, University of Ottawa, Canada.
  • Satoh M; Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University.
J Toxicol Sci ; 43(2): 89-100, 2018.
Article en En | MEDLINE | ID: mdl-29479038
Cadmium (Cd) is a common environmental pollutant with known toxic effects on the kidney. Urinary metabolomics is a promising approach to study mechanism by which Cd-induced nephrotoxicity. The aim of this study was to elucidate the mechanism of Cd toxicity and to develop specific biomarkers by identifying urinary metabolic changes after a long-term of Cd exposure and with the critical concentration of Cd in the kidney. Urine samples were collected from wild-type 129/Sv mice after 67 weeks of 300 ppm Cd exposure and analyzed by ultra performance liquid chromatography connected with quadrupole time of flight mass spectrometer (UPLC-QTOF-MS) based metabolomics approach. A total of 40 most differentiated metabolites (9 down-regulated and 31 up-regulated) between the control and Cd-exposed group were identified. The majority of the regulated metabolites are amino acids (glutamine, L-aspartic acid, phenylalanine, tryptophan, and D-proline) indicating that amino acid metabolism pathways are affected by long-term exposure of Cd. However, there are also some nucleotides (guanosine, guanosine monophosphate, cyclic AMP, uridine), amino acid derivatives (homoserine, N-acetyl-L-aspartate, N-acetylglutamine, acetyl-phenylalanine, carboxymethyllysine), and peptides. Results of pathway analysis showed that the arginine and proline metabolism, purine metabolism, alanine, aspartate and glutamate metabolism, and aminoacyl-tRNA biosynthesis were affected compared to the control. This study demonstrates that metabolomics is useful to elucidate the metabolic responses and biological effects induced by Cd-exposure.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Cadmio / Exposición a Riesgos Ambientales / Contaminantes Ambientales / Aminoácidos / Riñón Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Toxicol Sci Año: 2018 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Cadmio / Exposición a Riesgos Ambientales / Contaminantes Ambientales / Aminoácidos / Riñón Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Toxicol Sci Año: 2018 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Japón