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Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
Kononikhin, Alexey S; Sergeeva, Victoria A; Bugrova, Anna E; Indeykina, Maria I; Starodubtseva, Natalia L; Chagovets, Vitaliy V; Popov, Igor A; Frankevich, Vladimir E; Pedrioli, Patrick; Sukhikh, Gennady T; Nikolaev, Eugene N.
Afiliación
  • Kononikhin AS; Moscow Institute of Physics and Technology, Moscow, Russia. alex.kononikhin@gmail.com.
  • Sergeeva VA; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. alex.kononikhin@gmail.com.
  • Bugrova AE; V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia. alex.kononikhin@gmail.com.
  • Indeykina MI; Moscow Institute of Physics and Technology, Moscow, Russia.
  • Starodubtseva NL; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
  • Chagovets VV; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
  • Popov IA; V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
  • Frankevich VE; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
  • Pedrioli P; Moscow Institute of Physics and Technology, Moscow, Russia.
  • Sukhikh GT; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
  • Nikolaev EN; Moscow Institute of Physics and Technology, Moscow, Russia.
Methods Mol Biol ; 1719: 311-318, 2018.
Article en En | MEDLINE | ID: mdl-29476520
Urine is a sample of choice for noninvasive biomarkers search because it is easily available in large amounts and its molecular composition provides information on processes in the organism. The high potential of urine peptidomics has been demonstrated for clinical purpose. Several mass spectrometry based approaches have been successfully applied for urine peptidome analysis and potential biomarkers search. Summarizing literature data and our own experience we developed a protocol for comprehensive urine peptidome analysis. The technology includes several stages and consists of urine sample preparation by size exclusion chromatography and identification of featured peptides by nano-HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry, semiquantitative and statistical data analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Cromatografía Líquida de Alta Presión / Urinálisis / Ciclotrones / Espectroscopía Infrarroja por Transformada de Fourier / Espectrometría de Masa por Ionización de Electrospray Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Cromatografía Líquida de Alta Presión / Urinálisis / Ciclotrones / Espectroscopía Infrarroja por Transformada de Fourier / Espectrometría de Masa por Ionización de Electrospray Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos