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Sensing of Vimentin mRNA in 2D and 3D Models of Wounded Skin Using DNA-Coated Gold Nanoparticles.
Vilela, Patrick; Heuer-Jungemann, Amelie; El-Sagheer, Afaf; Brown, Tom; Muskens, Otto L; Smyth, Neil R; Kanaras, Antonios G.
Afiliación
  • Vilela P; Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, SO17 1BJ, UK.
  • Heuer-Jungemann A; Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, SO17 1BJ, UK.
  • El-Sagheer A; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
  • Brown T; Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez, 43721, Egypt.
  • Muskens OL; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
  • Smyth NR; Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, SO17 1BJ, UK.
  • Kanaras AG; Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Small ; 14(12): e1703489, 2018 03.
Article en En | MEDLINE | ID: mdl-29464860
Wound healing is a highly complex biological process, which is accompanied by changes in cell phenotype, variations in protein expression, and the production of active biomolecules. Currently, the detection of proteins in cells is done by immunostaining where the proteins in fixed cells are detected by labeled antibodies. However, immunostaining cannot provide information about dynamic processes in living cells, within the whole tissue. Here, an easy method is presented to detect the transition of epithelial to mesenchymal cells during wound healing. The method employs DNA-coated gold nanoparticle fluorescent nanoprobes to sense the production of Vimentin mRNA expressed in mesenchymal cells. Fluorescence microscopy is used to achieve temporal detection of Vimentin mRNA in wounds. 3D light-sheet microscopy is utilized to observe the dynamic expression of Vimentin mRNA spatially around the wounded site in skin tissue. The use of DNA-gold nanoprobes to detect mRNA expression during wound healing opens up new possibilities for the study of real-time mechanisms in complex biological processes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vimentina / ARN Mensajero / Nanopartículas del Metal Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vimentina / ARN Mensajero / Nanopartículas del Metal Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article Pais de publicación: Alemania