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The Involvement of Notch1-RBP-Jk/Msx2 Signaling Pathway in Aortic Calcification of Diabetic Nephropathy Rats.
Gong, Caipan; Li, Li; Qin, Chunmei; Wu, Weihua; Liu, Qi; Li, Ying; Gan, Linwang; Ou, Santao.
Afiliación
  • Gong C; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
  • Li L; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
  • Qin C; Department of Nephrology, Luzhou People's Hospital, Luzhou, Sichuan 646000, China.
  • Wu W; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
  • Liu Q; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
  • Li Y; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
  • Gan L; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
  • Ou S; Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
J Diabetes Res ; 2017: 8968523, 2017.
Article en En | MEDLINE | ID: mdl-29464183
BACKGROUND: This study explored the changes in expression of vascular smooth muscle cell (VSMC) markers and osteogenic markers, as well as the involvement of Notch1-RBP-Jk/Msx2 pathway in a rat model of diabetic nephropathy (DN) with vascular calcification. METHODS: A rat model of DN with concomitant vascular calcification was created by intraperitoneal injection of streptozotocin followed by administration of vitamin D3 and nicotine. Biochemical analysis and histological examination of aortic tissue were performed. VSMC markers and osteogenic markers as well as target molecules in Notch1-RBP-Jk/Msx2 were determined by quantitative real-time polymerase chain reaction and immunohistochemical analysis. RESULTS: Serum calcium and phosphorus levels were significantly increased in model rats as compared to that in normal controls. Diabetic rats with vascular calcification exhibited mineral deposits in aortic intima-media accompanied by decreased expression of VSMC markers and increased expression of osteogenic markers. Notch1, RBP-Jk, Msx2, Jagged1, and N1-ICD were barely expressed in the aortic wall of normal rats. In contrast, these were significantly increased in the model group at all time points (8, 12, and 16 weeks), as compared to that in the normal rats. CONCLUSION: Activation of the Notch1-RBP-Jk/Msx2 signaling pathway may be involved in the development and progression of vascular calcification in DN.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de la Aorta / Transducción de Señal / Proteínas de Homeodominio / Nefropatías Diabéticas / Proteína de Unión a la Señal Recombinante J de las Inmunoglobulinas / Receptor Notch1 / Calcificación Vascular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Diabetes Res Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de la Aorta / Transducción de Señal / Proteínas de Homeodominio / Nefropatías Diabéticas / Proteína de Unión a la Señal Recombinante J de las Inmunoglobulinas / Receptor Notch1 / Calcificación Vascular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Diabetes Res Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido