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An efficient biomarker panel for diagnosis of breast cancer using surface-enhanced laser desorption ionization time-of-flight mass spectrometry.
Yigitbasi, Turkan; Calibasi-Kocal, Gizem; Buyukuslu, Nihal; Atahan, Murat Kemal; Kupeli, Hakan; Yigit, Seyran; Tarcan, Ercument; Baskin, Yasemin.
Afiliación
  • Yigitbasi T; Department of Biochemistry, School of Medicine, Istanbul Medipol University, Istanbul 34810, Turkey.
  • Calibasi-Kocal G; Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir 35340, Turkey.
  • Buyukuslu N; Department of Nutrition and Dietetics, School of Health Sciences, Istanbul Medipol University, Istanbul 34810, Turkey.
  • Atahan MK; Department of General Surgery, Ataturk Training and Research Hospital, Katip Celebi University, Izmir 35360, Turkey.
  • Kupeli H; Department of General Surgery, Ataturk Training and Research Hospital, Katip Celebi University, Izmir 35360, Turkey.
  • Yigit S; Department of Pathology, Ataturk Training and Research Hospital, Katip Celebi University, Izmir 35360, Turkey.
  • Tarcan E; Department of General Surgery, Ataturk Training and Research Hospital, Katip Celebi University, Izmir 35360, Turkey.
  • Baskin Y; Department of Basic Oncology, Institute of Oncology, Dokuz Eylul University, Izmir 35340, Turkey.
Biomed Rep ; 8(3): 269-274, 2018 Mar.
Article en En | MEDLINE | ID: mdl-29456844
Breast cancer (BC) is the most frequently diagnosed cancer that affects women worldwide. Early detection of BC is important to improve survival rates and decrease mortality. The aim of the present study was to investigate serum biomarkers using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to distinguish patients with BC from the healthy population and patients with benign breast diseases (BBDs). A total of 62 patients with invasive ductal carcinoma, as confirmed by histopathology, and 47 non-cancerous individuals (NCIs) [16 healthy controls (HCs) and 31 patients with BBD] were enrolled in the present study. Serum protein profiles were determined by SELDI-TOF-MS using an immobilized metal affinity capture array. Serum from patients with BC were compared with that from the HC group using univariate and multivariate statistical analyses. A total of 118 clusters were generated from the individual serum. Univariate analysis revealed that 5 peaks were significantly downregulated (m/z 1,452, 2,670, 3,972, 5,354 and 5,523; P<0.001) and 4 were upregulated (m/z 6,850, 7,926, 8,115 and 8,143; P<0.001) in patients with BC compared with the HC group. A comparison of patients with BC and patients with BBD revealed an additional 9 protein peaks. Among these, 3 peaks (m/z 3,972, 5,336 and 11,185) were significantly downregulated and 6 peaks (m/z 4,062, 4,071, 4,609, 6,850, 8,115 and 8,133) were significantly upregulated. A total of 3 peaks [mass-to-change ratio (m/z) 3,972, 6,850 and 8,115 (BC2)] were common in both sets. The results of the present study suggest that a 4 protein peak set [m/z 3,972, 6,850 and 8,115 (BC2) and 8,949 (BC3)] could be used to distinguish patients with BC from NCI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Biomed Rep Año: 2018 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Screening_studies Idioma: En Revista: Biomed Rep Año: 2018 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido