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The overexpression of RXam1, a cassava gene coding for an RLK, confers disease resistance to Xanthomonas axonopodis pv. manihotis.
Díaz Tatis, Paula A; Herrera Corzo, Mariana; Ochoa Cabezas, Juan C; Medina Cipagauta, Adriana; Prías, Mónica A; Verdier, Valerie; Chavarriaga Aguirre, Paul; López Carrascal, Camilo E.
Afiliación
  • Díaz Tatis PA; Laboratorio Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá, Colombia.
  • Herrera Corzo M; Grupo de Ciencias Biológicas y Químicas, Departamento de Biología, Universidad Antonio Nariño, Cra1 #47a15, Bogotá, Colombia.
  • Ochoa Cabezas JC; Laboratorio Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá, Colombia.
  • Medina Cipagauta A; Programa de Biología y Mejoramiento de la Palma de Aceite, Cenipalma, Dir: Km 137 via Pto Araujo-La lizama, Bogotá, Colombia.
  • Prías MA; Laboratorio Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá, Colombia.
  • Verdier V; Department of Integrative Biology, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479, Poznan, Poland.
  • Chavarriaga Aguirre P; Plataforma de Transformación Genética, Centro Internacional de Agricultura Tropical (CIAT), Km 17 Recta Cali-Palmira, Palmira, Colombia.
  • López Carrascal CE; Plataforma de Transformación Genética, Centro Internacional de Agricultura Tropical (CIAT), Km 17 Recta Cali-Palmira, Palmira, Colombia.
Planta ; 247(4): 1031-1042, 2018 Apr.
Article en En | MEDLINE | ID: mdl-29453662
MAIN CONCLUSION: The overexpression of RXam1 leads to a reduction in bacterial growth of XamCIO136, suggesting that RXam1 might be implicated in strain-specific resistance. Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) is a prevalent disease in all regions, where cassava is cultivated. CBB is a foliar and vascular disease usually controlled through host resistance. Previous studies have found QTLs explaining resistance to several Xam strains. Interestingly, one QTL called XM5 that explained 13% of resistance to XamCIO136 was associated with a similar fragment of the rice Xa21-resistance gene called PCR250. In this study, we aimed to further identify and characterize this fragment and its role in resistance to CBB. Screening and hybridization of a BAC library using the molecular marker PCR250 as a probe led to the identification of a receptor-like kinase similar to Xa21 and were called RXam1 (Resistance to Xam 1). Here, we report the functional characterization of susceptible cassava plants overexpressing RXam1. Our results indicated that the overexpression of RXam1 leads to a reduction in bacterial growth of XamCIO136. This suggests that RXAM1 might be implicated in strain-specific resistance to XamCIO136.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Manihot / Xanthomonas axonopodis / Resistencia a la Enfermedad Tipo de estudio: Prognostic_studies Idioma: En Revista: Planta Año: 2018 Tipo del documento: Article País de afiliación: Colombia Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Manihot / Xanthomonas axonopodis / Resistencia a la Enfermedad Tipo de estudio: Prognostic_studies Idioma: En Revista: Planta Año: 2018 Tipo del documento: Article País de afiliación: Colombia Pais de publicación: Alemania