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Measles Virus Persistent Infection of Human Induced Pluripotent Stem Cells.
Naaman, Hila; Rabinski, Tatiana; Yizhak, Avi; Mizrahi, Solly; Avni, Yonat Shemer; Taube, Ran; Rager, Bracha; Weinstein, Yacov; Rall, Glenn; Gopas, Jacob; Ofir, Rivka.
Afiliación
  • Naaman H; 1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Rabinski T; 1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Yizhak A; 2 Regenerative Medicine & Stem Cell Research Center, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Mizrahi S; 3 Department of Surgery A, Soroka University Medical Center , Beer Sheva, Israel .
  • Avni YS; 3 Department of Surgery A, Soroka University Medical Center , Beer Sheva, Israel .
  • Taube R; 1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Rager B; 4 Department of Clinical Virology, Soroka University Medical Center , Beer Sheva, Israel .
  • Weinstein Y; 1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Rall G; 1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Gopas J; 1 The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva, Israel .
  • Ofir R; 5 Fox Chase Cancer Center , Blood Cell Development and Function, Philadelphia, Pennsylvania, USA.
Cell Reprogram ; 20(1): 17-26, 2018 02.
Article en En | MEDLINE | ID: mdl-29412740
In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Virus del Sarampión Límite: Animals / Humans Idioma: En Revista: Cell Reprogram Año: 2018 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Virus del Sarampión Límite: Animals / Humans Idioma: En Revista: Cell Reprogram Año: 2018 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos